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Heterogeneity of HeLa cell DNA as evidenced by CsCl density gradient centrifugation

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Summary

HeLa cell DNA was analyzed through CsCl density gradient centrifugation and thermal denaturation. Neutral centrifugation without ionic treatment allowed the resolution of a main peak with density 1.699 g/ml and of 3 satellites DNAs with densities 1.683, 1.710 and 1.720. First derivatives of melting curves showed the presence of 4 DNA families, whose G+C content calculated from Tm values corresponded almost exactly to the G+C content expected from the previously densities. The extraction method seems particularly suitable for quantitative separation of DNA classes.

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Author notes

  1. M. Durante

    Present address: Istituto di Genetica dell'Università, Via Matteotti 1/A, 56100, Pisa, Italy

Authors and Affiliations

  1. Centro Nucleare CAMEN, S. Piero a Grado, I-56100, Pisa, Italy

    M. Durante, Anna Covi & L. Barsanti

Authors
  1. M. Durante
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  2. Anna Covi
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  3. L. Barsanti
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Durante, M., Covi, A. & Barsanti, L. Heterogeneity of HeLa cell DNA as evidenced by CsCl density gradient centrifugation. Experientia 33, 1158–1159 (1977). https://doi.org/10.1007/BF01922301

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  • DOI: https://doi.org/10.1007/BF01922301

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