, Volume 50, Issue 5, pp 483–485 | Cite as

Inhibition of the sodium pump does not cause a stoichiometric decrease of ATP-production in energy limited fish hepatocytes

  • G. Krumschnabel
  • W. Wieser
Research Articles


In isolated goldfish hepatocytes underaerobic conditions the energy requirement for the sodium pump (calculated from Rb+ flux) is closely matched by the ouabain-sensitive fraction of oxygen consumption, whereas during in vitroanoxia (cyanide inhibition of the electron transport chain) the measured ATP demand of the sodium pump clearly exceeds ouabain-sensitive ATP production by anaerobic glycolysis. We conclude that when the energy status of cells is low, part or all of the ATP spared by the inhibition of a particular function may be used for fuelling other ATP-consuming functions.

Key words

Goldfish hepatocytes (Na+, K+)-ATPase protein synthesis membrane-metabolic coupling 


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Copyright information

© Birkhäuser Verlag 1994

Authors and Affiliations

  • G. Krumschnabel
    • 1
  • W. Wieser
    • 1
  1. 1.Institute of Zoology, Department of EcophysiologyUniversity of InnsbruckInnsbruck(Austria)

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