Experientia

, Volume 35, Issue 2, pp 247–248 | Cite as

Dose-effects of 8-methoxypsoralen and long wave UV-light in 3T3 cells: evaluation of a phototoxic index

  • J. Pohl
  • E. Christophers
Specialia

Summary

3T3 cells were cultured until confluency and treated with various doses of 8-methoxypsoralen followed by long wave UV light irradiation. The inhibition of3H-thymidine incorporation was dose-dependent for both, psoralen and light. A phototoxic index (PTI) was established demonstrating that a constant correlation between psoralen and UVA light exists for the photoinactivation in living cells.

Keywords

Living Cell Light Irradiation Psoralen Constant Correlation 

References

  1. 3.
    F. Dall'Acqua, S. Marciani, L. Ciavatta and G. Rodighiero, Z. Naturf.26b, 561 (1971).Google Scholar
  2. 4.
    R.S. Cole, Biochim. biophys. Acta217, 30 (1970).PubMedGoogle Scholar
  3. 5.
    R.S. Cole, Biochim. biophys. Acta254, 30 (1971).PubMedGoogle Scholar
  4. 6.
    F. Dall'Acqua, S. Marciani, D. Vedaldi, G. Rodighiero, Biochim. biophys. Acta353, 267 (1974).PubMedGoogle Scholar
  5. 7.
    M.A. Pathak and D.M. Krämer, Biochim. biophys. Acta195, 197 (1969).PubMedGoogle Scholar
  6. 8.
    E. Ben-Hur and M.M. Elkind, Biochim. biophys. Acta331, 181 (1973).PubMedGoogle Scholar
  7. 9.
    K. Wolff, H. Hönigsmann, F. Gschnait and K. Konrad, Dt. med. Wschr.100, 2471 (1975).Google Scholar
  8. 10.
    J.A. Parrish, T.B. Fitzpatrick, L. Tanenbaum and M.A. Pathak, New Engl. J. Med.291, 1207 (1974).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1979

Authors and Affiliations

  • J. Pohl
    • 1
  • E. Christophers
    • 1
  1. 1.Dept. of DermatologyUniversity of KielKiel(Federal Republic of Germany)

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