3T3 cells were cultured until confluency and treated with various doses of 8-methoxypsoralen followed by long wave UV light irradiation. The inhibition of3H-thymidine incorporation was dose-dependent for both, psoralen and light. A phototoxic index (PTI) was established demonstrating that a constant correlation between psoralen and UVA light exists for the photoinactivation in living cells.
Living Cell Light Irradiation Psoralen Constant Correlation
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F. Dall'Acqua, S. Marciani, L. Ciavatta and G. Rodighiero, Z. Naturf.26b, 561 (1971).Google Scholar