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Primary tissue cultures from organ fragments: A simplified method

Experientia volume 35, pages 181–182 (1979)Cite this article

Summary

Organ fragments washed in Ca++ and Mg++-free saline, treated with trypsin and placed directly into culture flasks adhered within seconds to the vessel surface. If the fragments were suspended in culture medium before they were added to the flasks, they did not adhere. This technique permits the rapid attachment and subsequent growth of the primary tissue cultures.

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Author information

Affiliations

  1. Department of Biological Sciences, Florida Atlantic University, 33431, Boca Raton, Florida, USA

    E. J. Noga

Authors
  1. E. J. Noga
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Additional information

Adapted from a thesis submitted in partial fulfillment of the requirements for the degree of Master of Science at Florida Atlantic University.

Acknowledgment. This work was carried out in the laboratory of J.X. Hartmann, whom I thank for his generosity and helpful discussion.

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Noga, E.J. Primary tissue cultures from organ fragments: A simplified method. Experientia 35, 181–182 (1979). https://doi.org/10.1007/BF01920601

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  • DOI: https://doi.org/10.1007/BF01920601

Keywords

  • Tissue Culture
  • Trypsin
  • Culture Flask
  • Subsequent Growth
  • Primary Tissue