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Hydrogen peroxide generation inTrypanosoma cruzi

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Summary

Homogenates from T. cruzi epimastigotes produced 3.4 pmoles H2O2/min 106 cells, as detected by the cytochrome c peroxidase assay. Addition of NADH or NADPH increased H2O2 production by a factor of 3 and 5, respectively. When supplemented with NADH and NADPH, the mitochondrial, microsomal, and supernatant fractions produced H2O2, the soluble fraction and the mitochondrial membranes being apparently the main generators of H2O2. The epimastigote homogenates showed cyanide-sensitive superoxide dismutase activity, equivalent to 0.28 μg bovine superoxide dismutase per mg homogenate protein.

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Authors and Affiliations

  1. Facultad de Medicina, Instituto de Química Biológica, Universidad de Buenos Aires, Paraguay. 2155, 1121 CF, Buenos Aires, Argentina

    A. Boveris & A. O. M. Stoppani

Authors
  1. A. Boveris
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  2. A. O. M. Stoppani
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Additional information

This investigation was supported by grants from Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET) Argentina and the Scientific Office, American States Organization.

Career Investigator of CONICET.

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Boveris, A., Stoppani, A.O.M. Hydrogen peroxide generation inTrypanosoma cruzi. Experientia 33, 1306–1308 (1977). https://doi.org/10.1007/BF01920148

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