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The antiviral effect of Keishi-ni-eppi-ichi-to, a tranditional Chinese herbal medicine, on influenza A2(H2N2) virus infection in mice

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Abstract

The antiviral effect of Keishi-ni-eppi-ichi-to (TJS-064), a traditional Chinese herbal medicine, was investigation in mice infected with influenza A2(H2N2) virus. When mice exposed to 5 LD50 dose of the virus were treated orally with a 70 mg/kg dose of TJS-064 1 day before and 1 day and 4 days after the infection, 100% survived over a 25-day experimental period. At the end of this period all the control mice, treated with saline alone, had died; their mean survival time in days (MSD) was 11.2 days. When mice infected with a 10 LD50 dose of the virus were treated with TJS-064, the MSD was >17.4 days and there was a 50% survival rate, while the control group had a MSD of 8.7 days and 0% survival rate. No significant antiviral effect TJS-064 was observed when the agent was administered orally to mice infected with a 100 LD50 or large dose of influenza virus. Pulmonary consolidation, virus titers in lung tissues and HAI titers in sera of infected mice treated with TJS-064 were all significantly lower than those of infected mice treated with saline. Interferon activities were detected in sera of mice treated with the agent at a dose of 100 mg/kg orally. Since viricidal and viristatic activities of the agent against influenza virus were not demonstrated, the antiviral effects of TJS-064 may be expressed through the host's antiviral functions including interferon production.

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Authors and Affiliations

  1. Department of Internal Medicine, University of Texas Medical Branch, 77555, Galveston, Texas, USA

    M. A. Ball, T. Utsunomiya, K. Ikemoto, M. Kobayashi, R. B. Pollard & F. Suzuki

  2. Shriners Burns Institute, 77555, Galveston, Texas, USA

    M. Kobayashi & F. Suzuki

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  1. M. A. Ball
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  2. T. Utsunomiya
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  3. K. Ikemoto
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  4. M. Kobayashi
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  5. R. B. Pollard
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  6. F. Suzuki
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Ball, M.A., Utsunomiya, T., Ikemoto, K. et al. The antiviral effect of Keishi-ni-eppi-ichi-to, a tranditional Chinese herbal medicine, on influenza A2(H2N2) virus infection in mice. Experientia 50, 774–779 (1994). https://doi.org/10.1007/BF01919381

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