Skip to main content
SpringerLink
Log in

Molecular analysis of a candidate gene for the reproductive isolation between sibling species ofDrosophila

  • Research Articles
  • Published:
Experientia Aims and scope Submit manuscript

Abstract

The X-linked geneHmr inDrosophila melanogaster, when mutated, rescues otherwise inviable interspecific hybrids from crosses betweenD. melanogaster and any of its three most closely related speciesD. simulans, D. mauritiana andD. sechellia. DNA from the site of a breakpoint at the putative locus of the gene has been cloned, and results of transcription and sequence analyses are presented. Three distinct mRNAs are transcribed from this locus, two of which are abundantly expressed throught life. A third transcript, which is larger but rarer, appears to be disrupted by at least one of the two known mutations ofHmr. The gene encodes a mitochondrial ADP/ATP translocator protein, which plays an essential role in maintaining metabolic energy. Analysis of several cDNAs suggested that the rescue of hybrids may be dependent on mutations in the variable 3′ end region of this gene, affecting the level and/or the stability of the largest messenger RNA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Dobzhansky, T., Genetics and the Origin of Species. Columbia University Press, New York 1937.

    Google Scholar 

  2. Muller, H. J., in: The New Systematics, p. 185. Ed. J. S. Huxley. Clarendon Press, Oxford 1940.

    Google Scholar 

  3. Muller, H. J., Biol. Symp.6 (1942) 71.

    Google Scholar 

  4. Mayr, E., Systematics and the Origin of Species. Columbia University Press, New York 1942.

    Google Scholar 

  5. Zouros, E., Genetics97 (1981) 703.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Coyne, J. A., and Charlesworth, B., Heredity57 (1986) 243.

    Article  PubMed  Google Scholar 

  7. Pantazidis, A. C., and Zouros, E., Heredity60 (1988) 299.

    Article  PubMed  Google Scholar 

  8. Orr, H. A., Genet. Res.59 (1992) 73.

    Article  CAS  PubMed  Google Scholar 

  9. Perez, D. E., Wu, C.-I., Johnson, N. A., and Wu, M.-L., Genetics133 (1993) 261.

    Article  Google Scholar 

  10. Guenet, J.-L., Nagamine, C., Simon-Chazottes, D., Montagutelli, X., and Bonhomme, F., Genet. Res.56 (1990) 163.

    Article  CAS  PubMed  Google Scholar 

  11. Macnair, M., and Christie, P., Heredity50 (1983) 295.

    Article  CAS  Google Scholar 

  12. Wittbrodt, J., Adam, D., Malitschek, B., Maueler, W., Raulf, F., Telling, A., Robertson, S. M., and Schartl, M., Nature341 (1989) 415.

    Article  CAS  PubMed  Google Scholar 

  13. Cabot, E. L., Davis, A. W., Johnson, N. A., and Wu, C.-I., Genetics137 (1994) 175.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Wu, C.-I., and Davis, A. W., Am. Nat.142 (1993) 187.

    Article  CAS  PubMed  Google Scholar 

  15. Coyne, J. A., and Orr, H. A., Evolution43 (1989) 362.

    Article  PubMed  Google Scholar 

  16. Haldane, J. B. S., J. Genet.12 (1922) 101.

    Article  Google Scholar 

  17. Hertwig, P., in: Artbastarde bei Tieren (Handbuch der Vererbungswissenschaft, Band II, B), p. 140. Ed. E. Baur and M. Hartmann. Gebrüder Bornträger, Berlin 1936.

    Google Scholar 

  18. Bock, I. R., Evol. Biol.18 (1984) 41.

    Article  Google Scholar 

  19. Sturtevant, A. H., Genetics5 (1920) 488.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Sturtevant, A. H., Genetics6 (1921) 43.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Hadorn, E., Revue suisse Zool.68 (1961) 197.

    Article  Google Scholar 

  22. Orr, H. A., Nature361 (1993) 532.

    Article  CAS  PubMed  Google Scholar 

  23. Sperlich, D., Drosoph. Inf. Serv.36 (1962) 118.

    Google Scholar 

  24. Mensua, J. L., and Perez, M., Drosoph. Inf. Serv.52 (1977) 60.

    Google Scholar 

  25. Watanabe, T. K., Jap. J. Genet.54 (1979) 325.

    Article  Google Scholar 

  26. Sawamura, K., Taira, T., and Watanabe, T. K., Genetics133 (1993) 299.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Hutter, P., and Ashburner, M., Nature327 (1987) 331.

    Article  CAS  PubMed  Google Scholar 

  28. David, J. R., Lemeunier, F., Tsacas, L., and Bocquet, C., Ann. Genet.17 (1974) 235.

    CAS  PubMed  Google Scholar 

  29. Lachaise, D., David, J. R., Lemeunier, F., Tsacas, L. and Ashburner, M., Evolution40 (1986) 262.

    PubMed  Google Scholar 

  30. Hutter, P., Roote, J., and Ashburner, M., Genetics124 (1990) 909.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Sawamura, K., Yamamoto, M.-T., and Watanabe, T. K., Genetics133 (1993) 307.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Sawamura, K., and Yamamoto, M.-T., Molec. gen. Genet.239 (1993) 441.

    Article  CAS  PubMed  Google Scholar 

  33. Naveira, H. F., Heredity68 (1992) 211.

    Article  PubMed  Google Scholar 

  34. Davis, A. W., Noonburg, E. G., and Wu, C.-I., Genetics137 (1994) 191.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Pontecorvo, G., J. Genet.45 (1943) 51.

    Article  Google Scholar 

  36. Yamamoto, M. T., Genetica87 (1992) 151.

    CAS  PubMed  Google Scholar 

  37. Hutter, P., Genet. Res.55 (1990) 159.

    Article  CAS  PubMed  Google Scholar 

  38. Zhimulev, I. F., Pokholkova, G. V., Bgatov, A. B., Umbetova, G. H., Solovjeva, I. V., Khudyakov, Yu. E., and Belyaeva, E. S., Biol. Zbl.106 (1987) 699.

    Google Scholar 

  39. Johnson, M. M., Woloshyn, E. P., and Nash, D., Molec. gen. Genet.174 (1979) 287.

    Article  CAS  PubMed  Google Scholar 

  40. Frischauf, A.-M., Lehrach, H., Poustka A., and Murray, N., J. molec. Biol.170 (1983) 827.

    Article  CAS  PubMed  Google Scholar 

  41. Benton, W. D., and Davis, R. W., Science196 (1977) 180.

    Article  CAS  PubMed  Google Scholar 

  42. Bender, W., Spierer, P., and Hogness, D. S., J. molec. Biol.168 (1983) 17.

    Article  CAS  PubMed  Google Scholar 

  43. Davis, L. G., Dibner, M. D. and Battey, J. F., Basic Methods in Molecular Biology. Elsevier. Amsterdam-New York 1986.

    Google Scholar 

  44. Southern, E., J. molec. Biol.98 (1975) 503.

    Article  CAS  PubMed  Google Scholar 

  45. Khandjian E. W., Bio/Technology5 (1987) 165.

    CAS  Google Scholar 

  46. Girvitz, S. C., Bacchetti, S., Rainbow, R. J., and Graham, F. L., Analyt. Biochem.106 (1980) 492.

    Article  CAS  PubMed  Google Scholar 

  47. Feinberg, A. P., and Vogelstein, B., Analyt. Biochem.137 (1984) 266.

    Article  CAS  PubMed  Google Scholar 

  48. Maniatis, T. E., Fritsch F., and Sambrook, J., Molecular cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York 1982.

    Google Scholar 

  49. Sanger, F., Coulson, A. R., Barrel, B. G., Smith, A. J. H., and Roe, B. A., J. molec. Biol.143 (1980) 161.

    Article  CAS  PubMed  Google Scholar 

  50. Pardue, M. L., and Gall, J. G., in: Methods in Cell Biology, vol. 10. Ed. D. Prescot. Academic Press, New York, 1975.

    Google Scholar 

  51. Engels, W. R., Preston, C. R., Thompson, P., and Eggleston, W. B., Focus8 (1986) 6.

    Google Scholar 

  52. Lindsley, D. L., and Zimm, G. C. The Genome of Drosophila melanogaster. Academic Press, San, Diego 1992.

    Google Scholar 

  53. Maniatis, T., Hardison, R. C., Lacy, E., Lauer, J., O'Connell, C., Quon, D., Sim, G. K., and Efstratiadis, A., Cell15 (1978) 687.

    Article  CAS  PubMed  Google Scholar 

  54. Sidén-Kiamos I., Saunders, R. D. C., Spanos, L., Majerus, T., Treaner, J., Savakis, C., Louis, C., Glover, D. M., Ashburner, M., and Kafatos F. C. Nucl. Acids Res.18 (1990) 6261.

    Article  PubMed  PubMed Central  Google Scholar 

  55. Proudfoot, N. J., and Brownlee, G. G., Nature263 (1976) 211.

    Article  CAS  PubMed  Google Scholar 

  56. Kolarov, J., Kolarova, N., and Nelson, N., J. biol. Chem.265 (1990) 12711.

    Article  CAS  PubMed  Google Scholar 

  57. Louvi, A., and Tsitilou, S. G., J. molec. Evol.35 (1992) 44.

    Article  CAS  PubMed  Google Scholar 

  58. Battini, R., Ferrari, S., Kaczmareck, L., Calabretta, B., Chen, S., and Baserga, R., J. biol. Chem.262 (1987) 4355.

    Article  CAS  PubMed  Google Scholar 

  59. Houldsworth, J., and Attardi, G., Proc. natl Acad. Sci.85 (1988) 377.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  60. Neckelmann, N., Li, K., Wade, R. P., Shuster, R., and Wallace, D. C., Proc. natl Acad. Sci.84 (1987) 7580.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  61. Li, K., Warner, C. K., Hodge, J. A., Minoshima, S., Kudoh, J., Fukuyama, R., Maekawa, M., Shimizu, Y., Shimizu, N., and Wallace, D. C., J. biol. Chem.264 (1989) 13998.

    Article  CAS  PubMed  Google Scholar 

  62. Adrian, G. S., McCammon, M. T., Montgomery, D. L., and Douglas, M. G., Molec. cell. Biol.6 (1986) 626.

    CAS  PubMed  PubMed Central  Google Scholar 

  63. Lefèvre, G. Jr., Genetics67 (1971) 497.

    Article  PubMed  PubMed Central  Google Scholar 

  64. Homyk, T., Szidonya, J. J., and Suzuki, D. T., Molec. gen. Genet.177 (1980) 553.

    Article  PubMed  Google Scholar 

  65. Powell, S. J., Medd, S. M., Runswick, M. R., and Walker, J. E., Biochemistry28 (1989) 866.

    Article  CAS  PubMed  Google Scholar 

  66. Saraste, M., and Walker, J. E., FEBS Lett.144 (1982) 250.

    Article  CAS  PubMed  Google Scholar 

  67. Patterson, J. T., and Griffen, A. B., Univ. Texas Publ.4445 (1944) 212.

    Google Scholar 

  68. Kaufmann, B. P., J. Morph.66 (1940) 197.

    Article  Google Scholar 

  69. Orr, H. A., Evolution43 (1989) 180.

    Article  PubMed  Google Scholar 

  70. Kondo, R., Matsuura, E. T., and Chigusa, S. I., Genet. Res.59 (1992) 81.

    Article  CAS  PubMed  Google Scholar 

  71. Wallace, D. C., in: Medical and Experimental Mammalian Genetics: A Perspective, vol. 23, p. 137. Eds V. A., McKusick, T. H. Roderick, J. Mori, and N. W. Paul. Liss, New York 1987.

    Google Scholar 

  72. Garesse, R., Genetics118 (1988) 649.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  73. Polan, M. L., Friedman, S., Gall, J. G., and Gehring, W. J., J. cell. Biol.56 (1988) 580.

    Article  Google Scholar 

  74. Klingenberg, M., Nature290 (1981) 449.

    Article  CAS  PubMed  Google Scholar 

  75. Hutter P., Genetica72 (1987) 193.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Génétique, Université de Genève, 154 route de Malagnou, Ch-1224, Genève, Switzerland

    P. Hutter & F. Karch

Authors
  1. P. Hutter
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Karch
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hutter, P., Karch, F. Molecular analysis of a candidate gene for the reproductive isolation between sibling species ofDrosophila. Experientia 50, 749–762 (1994). https://doi.org/10.1007/BF01919377

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01919377

Key words

Search

Navigation