Skip to main content
Log in

Geographic populations of the medfly may be differentiated by mitochondrial DNA variation

  • Research Articles
  • Published:
Experientia Aims and scope Submit manuscript


Restriction enzyme cleavage sites of mitochondrial DNA (mtDNA) from the Mediterranean fruit fly were found to vary among introduced populations in the Neotropics. The survey included samples from 15 established natural populations and 5 laboratory cultures from Hawaii, Central America, South America and West Africa and samples from recent California infestations (1989, 1991). Based on restriction fragment length polymorphisms from 2 enzymes, Hawaii is an unlikely source for the 1989 and 1991 California infestations. Interpopulational variation in mtDNA demonstrates the potential for the technique to trace the process of colonization (geographic spread) by this insect.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others


  1. Quaintance, A. L., U.S.D.A. Ent. Circ. No. 160 (1912) 25p.

  2. Quayle, H. J., Univ. Calif. agr. Exp. Stn Circ. No. 315 (1929) 19p.

  3. Christenson, L. D., and Stone, W. E., California Citrograph41 (1956) 159.

    Google Scholar 

  4. Clark, R. A., and Weems Jr, H. V., Proc. Fla State Hort. Soc.102 (1989) 159.

    Google Scholar 

  5. Miller, C. E., A Mediterranean Fruit Fly Risk Assessment, USDA, APHIS, PPD 1991, 106 p.

  6. Carey, J. R. Science253 (1991) 1369.

    Article  CAS  PubMed  Google Scholar 

  7. Saul, S. H., Science255 (1992) 515.

    Article  CAS  PubMed  Google Scholar 

  8. Kourti, A., Loukas, M., and Economopolous, A. P., in: Genetic Sexing of the Mediterranean Fruit Fly. p. 7. IAEA, Panel Proceedings Services, Vienna 1990.

    Google Scholar 

  9. Smith, D. R., Brown, W. M., and Taylor, O. R., Nature321 (1989) 674.

    Article  Google Scholar 

  10. Steck, G. J., and Sheppard, W. S., in: Fruit Flies of Economic Importance. Proc. Eds P. Liedo and M. Aluja 1991, in press.

  11. Sheppard, W. S., Rinderer, T. E., Mazzoli, J., Stelzer, J. A., and Shimanuki, H., Nature,349 (1991) 782.

    Article  Google Scholar 

  12. Maniatis, T., Fritsch, E. F., and Sambrook, J., Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, 1982.

  13. Marusyk, R., and Sergeant, A., Analyt. Biochem.,105 (1980) 403.

    Article  CAS  PubMed  Google Scholar 

  14. Azerodo-Espin, A. M. L., Schroder, R. F. W., Huettel, M. D., and Sheppard, W. S., Experientia47 (1991) 483.

    Article  Google Scholar 

  15. Snedecor, G. W., and Cochran, W. G., Statistical Methods. Iowa State Univ. Press. 1967.

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and permissions

About this article

Cite this article

Sheppard, W.S., Steck, G.J. & McPheron, B.A. Geographic populations of the medfly may be differentiated by mitochondrial DNA variation. Experientia 48, 1010–1013 (1992).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:

Key words