Summary
Adrenal medullary and retinal myosins formed bipolar filaments in vitro. These filaments showed features suggesting flexibility in the rod region of the myosin molecules composing such filaments; in certain cases the myosin heads spread away from the filament backbone, in others the backbone itself was twisted. In addition the bare central backbone showed transverse striations.
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We thank G. Devilliers for assistance with electron microscopy, D. Thiersé for technical assistance during purification of adrenal medulla myosin and Professor P. Mandel for his interest and support J.E.H. gratefully acknowledges receipt of a Royal Society European Exchange Fellowship and a grant from INSERM. N.V. is chargée de recherches au CNRS, D.A. is chargé de recherches à l'INSERM.
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Hesketh, J.E., Virmaux, N. & Aunis, D. Some observations on bipolar filaments formed by non-muscle myosins. Experientia 35, 31–33 (1979). https://doi.org/10.1007/BF01917858
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DOI: https://doi.org/10.1007/BF01917858