Abstract
A differential scanning calorimetric, thermogravimetric and electron microscopic investigation has been carried out on the uncalcified areas of turkey leg flexor tendon as a function of age. Rehydrated samples exhibit an increase of thermal stability with age. The δHD values drop from about 11 cal·g−1 in the first weeks of life down to 7 cal·g−1 after the 11th week.
At about 11 weeks, the collagen fibril diameter distribution passes from unimodal to multimodal. The DSC curves as well as the TG-DTG curves recorded from dried samples do not show any appreciable difference with ageing. The variations in thermal behaviour of rehydrated samples and fibril diameter distribution could be related to modifications in water binding with ageing.
Zusammenfassung
Mittels DSC, TG und Elektronenmikroskop wurden unverkalkte Beugesehnenregionen von Truthahnbeinen in Abhängigkeit des Alters untersucht. Rehydratierte Proben zeigen mit zunehmendem Alter ein Ansteigen der thermischen Stabilität. Der Wert von δHD sinkt von 11 cal/g in den ersten Lebenswochen auf 7 cal/g nach der 11.Woche.
Nach etwa 11 Wochen geht der Durchmesser der Kollagenfibrillen vom einer eingipfeligen in eine multigipfelige Verteilung über. Weder die DSC-, noch die TG-DTG-Kurven von getrockneten Proben zeigen irgendwelche nennenswerten Unterschiede in Abhängigkeit vom Alter. Die änderungen des thermischen Verhaltens rehydratierter Proben und der Verteilung des Fibrillendurchmessers können den änderungen der Bindung des Wassers in Abhängigkeit vom Alter zugeschrieben werden.
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The Authors are grateful to Dr. G. Fabris for discussion and help in the selection of the samples. They also wish to thank Mr. G. Pizzuto for excellent technical assistance. The financial support by Consiglio Nazionale delle Ricerche is gratefully acknowledged.
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Bigi, A., Castellani, P.P., Cojazzi, G. et al. Age related changes in the thermal transition of Turkey leg flexor tendon collagen. Journal of Thermal Analysis 38, 505–514 (1992). https://doi.org/10.1007/BF01915515
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DOI: https://doi.org/10.1007/BF01915515