Skip to main content
SpringerLink
Log in

Reoxidation of reduced hen egg white lysozyme fragment 1–123

  • Specialia
  • Published:
Experientia Aims and scope Submit manuscript

Summary

The reactivation of reduced lysozyme, whose 6 COOH-terminal amino acid including cysteine 127 were cut off, was studied. The results show that the disulfide bridge I–VIII as well as the COOH-terminal hexapeptide do not play a decisive role in the acquisition of the native 3-dimensional structure of the enzyme.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. D.F. Steiner and J.L. Clark, Proc. nat. Acad. Sci. USA60, 622 (1968).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. J.C. Brown, H.E. Swaigood and H.R. Horton, Biochem. biophys. Res. Commun.48, 1068 (1972).

    Article  CAS  PubMed  Google Scholar 

  3. D. Givol, F. De Lorenzo, R.F. Goldberger and C.B. Anfinsen, Proc. nat. Acad. Sci. USA53, 676 (1965).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. G.H. Dixon and A.C. Warlaw, Nature188, 721 (1960).

    Article  CAS  PubMed  Google Scholar 

  5. I. Kato and C.B. Anfinsen, J. biol. Chem.244, 1004 (1969).

    Article  CAS  PubMed  Google Scholar 

  6. H. Taniuchi, J. biol. Chem.245, 5459 (1970).

    Article  CAS  PubMed  Google Scholar 

  7. H. Taniuchi and C.B. Anfinsen, J. biol. Chem.243, 4778 (1968).

    Article  CAS  PubMed  Google Scholar 

  8. H. Taniuchi and C.B. Anfinsen, J. biol. Chem.244, 3864 (1969).

    Article  CAS  PubMed  Google Scholar 

  9. E.R. Johnson, K.J. Oh and D.B. Wetlaufer, J. biol. Chem.251, 3154 (1976).

    Article  CAS  PubMed  Google Scholar 

  10. W.L. Anderson and D.B. Wetlaufer, J. biol. Chem.251, 3147 (1976).

    Article  CAS  PubMed  Google Scholar 

  11. R. Hayashi, S. Moore and W.H. Stein, J. biol. Chem.248, 2296 (1973).

    Article  CAS  PubMed  Google Scholar 

  12. R.W. Kuhn, K.A. Walsh and H. Neurath, Biochemistry13, 3871 (1974).

    Article  CAS  PubMed  Google Scholar 

  13. S.S. Ristow and D.B. Wetlaufer, Biochem. biophys. Res. Commun.50, 544 (1973).

    Article  CAS  PubMed  Google Scholar 

  14. D. Spackman, W.H. Stein and S. Moore, Analyt. Chem.30, 1190 (1958).

    Article  CAS  Google Scholar 

  15. J. Holles, J. Jauregui-Adell, I. Bernier and P. Jolles, Biochim. biophys. Acta78, 668 (1963).

    Article  Google Scholar 

  16. A.J. Sophianopoulos, C.K. Rhodes, D.N. Holcomb and K.E. Van Holde, J. biol. Chem.237, 1107 (1962).

    Article  CAS  PubMed  Google Scholar 

  17. A.F.S.A. Habeeb, Meth. Enzym.25, 455 (1976).

    Google Scholar 

  18. E. Polastro, Y. Looze and J. Léonis, Biochim. biophys. Acta446, 310 (1976).

    Article  CAS  PubMed  Google Scholar 

  19. V.P. Saxena and D.B. Wetlaufer, Biochemistry9, 5015 (1970).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Chimie Générale I, Faculté des Sciences, Université libre de Bruxelles, 50, Avenue F.D. Roosevelt, B-1050, Bruxelles, Belgium

    Y. Looze, A. Vizet, J. P. Perraudin, J. Depreter, M. Deconinck, A. G. Schnek & J. Léonis

Authors
  1. Y. Looze
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Vizet
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. P. Perraudin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Depreter
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Deconinck
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. G. Schnek
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. Léonis
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Acknowledgments. The authors are grateful to Miss B. Couteaux for her help with regard to the preparation of carboxypeptidase Y. This work was supported by a Grant of the Fonds National Belge de la Recherche Fondamentale et Collective and by a grant of the Fonds Emile Defay.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Looze, Y., Vizet, A., Perraudin, J.P. et al. Reoxidation of reduced hen egg white lysozyme fragment 1–123. Experientia 34, 1017–1018 (1978). https://doi.org/10.1007/BF01915319

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01915319

Keywords

Search

Navigation