Summary
Callus tissue ofVicia faba has been grown from explants of immature embryos. The karyological analysis by the Giemsa C-banding technique has shown triploid and tri-aneuploid mitoses, all with an extra chromosome and with structurally altered karyotypes. Estimates of the amounts of interphase heterochromatin (chromocentres) indicate endopolyploidy up to 12 n.
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This work was financially supported by the Research Council of the S.R. Croatia — SIZIV. The authors wish to express their gratitude to the Industroprojekt Zagreb for the use of the image analyser, to Mr M. Denih for the area determinations of drawings, and to Dr D. Schweizer, Vienna, for critical comments.
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Papeš, D., Jelaska, S., Tomaseo, M. et al. Triploidy in callus culture ofVicia faba L. investigated by the Giemsa C-banding technique. Experientia 34, 1016–1017 (1978). https://doi.org/10.1007/BF01915318
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DOI: https://doi.org/10.1007/BF01915318