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Ca2+-uptake and-release phenomena from cardiac mitochondria under normal and ischemic conditions

Ca2+-Aufnahme-und-Freisetzungsphänomene kardialer Mitochondrien unter Normalbedingungen und nach Ischämie

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Summary

Guinea-pig and dog heart mitochondria were isolated in a KEA-medium. Ca2+-transport across mitochondrial membranes was measured continuously with an Aminco Dual-Wavelength-Spectrophotometer and murexide as a Ca2+-sensitive indicator. Ischemia was produced by cardioplegia at 15°C according toBretschneider. Guinea-pig heart mitochondria as well as mitochondria from dog heart show a spontaneous Ca2+-release without nonphysiological influence. Addition of 3.5 M Na+ can induce a very quick release of Ca2+ taken up by heart mitochondria. This release is different from that occurring spontaneously. Progressive ischemia results in a marked depression of Ca2+-uptake and spontaneous Ca2+-release.

Zusammenfassung

Meerschweinchen- und Hundeherzmitochondrien wurden in einem KEA-Medium isoliert und der mitochondriale Ca2+-Transport kontinuierlich mit einem Aminco-DW-2-Spektrophotometer und Murexid als Indikator gemessen. Ebenso wie bei Meerschweinchenherzmitochondrien läßt sich eine spontane Ca2+-Freisetzung auch bei Mitochondrien aus Hundeherzen zeigen. Ein anderer Typ der Ca2+-Freisetzung läßt sich durch Zugabe von 3,5 mM Na+ induzieren. Mit zunehmender ischämischer Myokardzellschädigung nimmt die mitochondriale Ca2+-Aufnahme und die spontane Freisetzung beträchtlich ab.

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References

  1. Göring, G. G., W. G. Nayler, P. G. Spieckermann Basic. Res. Cardiol.72, 77–81(1977)

    Article  PubMed  Google Scholar 

  2. Göring, G. G., H. Kahles, N. Nordbeck, C. J. Preuße, P. G. Spieckermann: Pflügers Arch.365, R3 (1976).

    Google Scholar 

  3. Langer, G. A.: Fed. Proc.35, 1274–1278 (1976).

    PubMed  Google Scholar 

  4. Scarpa, A., P. Graziotti: J. Gen. Physiol.62, 756–772 (1973).

    Article  PubMed  Google Scholar 

  5. Bretschneider, H. J., G. Hübner, D. Knoll, H. Lohr, H. Nordbeck, P. G. Spiekkermann: J. Cardiovasc. Surg.16, 241–260 (1975).

    Google Scholar 

  6. Sohrdahl, L. A., C. Johnson, Z. R. Blailock., A. Schwartz: Methods in Pharmacology, Vol. 1, ed.Schwartz, A. (New York 1971).

  7. Borle, A. A.: J. Membrane Biol.16, 221–236 (1974).

    Article  Google Scholar 

  8. Scarpa, A., K. Malmstrom, M. Chiesi, E. Carafoli J. Membrane Biol.29, 205–208 (1976).

    Article  Google Scholar 

  9. Carafoli, E., R. Tozzo, G. Lugli, F. Crovetti, C. Kratzing: J. Mol. Cell. Cardiol.6, 361–371 (1974).

    Article  PubMed  Google Scholar 

  10. Crompton, M., M. Campano, E. Carafoli: Febs Symposium Zürich, P. 417 (1976).

  11. Sordahl, S. A.: Arch. Biochem. Biophys.,167, 104–115 (1974).

    Article  Google Scholar 

  12. Puskin, J. S., T. E. Gunter, K. K. Gunter, P. R. Russel: Biochemistry15, 3834–3842 (1976).

    Article  PubMed  Google Scholar 

  13. Göring, G. G., P. G. Spieckermann: in preparation.

  14. Kahles, H., G. G. Göring, H. Nordbeck, C. J. Preuße andP. G. Spieckermann: Basic Res. Cardiol. (in press).

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Author notes

  1. G. G. Göring &  P. G. Spieckermann

    Present address: Physiologisches Institut, Lehrstuhl I, Abteilung Herzstoffwechsel, Universität Göttingen, Humboldtallee 7, D-3400, Göttingen

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    1. G. G. Göring
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    2. P. G. Spieckermann
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    Additional information

    With 4 figures

    Supported by the Deutsche Forschungsgemeinschaft-SFB 89-Kardiologie, Göttingen

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    Göring, G.G., Spieckermann, P.G. Ca2+-uptake and-release phenomena from cardiac mitochondria under normal and ischemic conditions. Basic Res Cardiol 73, 126–132 (1978). https://doi.org/10.1007/BF01914660

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    • DOI: https://doi.org/10.1007/BF01914660

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