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Axoplasmic organelles: Quantitative differences between ventral and dorsal root fibres of the rat

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Zusammenfassung

Motorische und sensible Nervenfasern lassen sich im Bereich der Rückenmarkswurzeln (Ratte, L5) aufgrund der Mengenverteilung der Axonorganellen unterscheiden. Bei vergleichbarem Faserkaliber haben Vorderwurzelfasern signifikant mehr Neurotubuli und axoplasmatisches Retikulum als Hinterwurzelfasern.

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References

  1. H. Schmidt andR. Stämpfli, Helv. physiol. Acta22, C143 (1964).

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  2. W. Zenker, 66. Vers. Anat. Ges., Zagreb 1971, Anat. Anz., in press.

  3. The ventral and dorsal roots of the 5th lumbar segment of 3 albino rats (Sprague-Dawley/300–350g) were used. Fixation by perfusion with phosphate-buffered glutaraldehyde under nembutal anaesthesia; preparation of the roots and postfixation in OsO4; embedding in epon; double staining of the ultrathin sections with uranyl acetate and lead citrate; Zeiss EM 9S electron microscope. Micrographs of complete nerve fibre cross sections were made at standard magnifications of ×5,000; for morphometric measurements prints at ×15,000 were used. Samples of 10 nerve fibre cross sections from both the A-α and the A-γ peaks of all 3 ventral roots and from comparable diameter classes of the 3 dorsal roots, as well as from a class of very small myelinated fibres of the dorsal roots, were used for evaluation. The following parameters have been determined and subjected to statistical analysis: 1. area of axon cross sections in μm2 (planimetry of the micrographs), 2. percent amount of mitochondria on axonal cross sections (morphometry by differential point counting). 3. percent amount of axoplasmic reticulum (differential point counting). 4. total counts of neurotubules per axon cross section and calculation of the densities (number per μm2), and 5. counts of neurofilaments on the prints in 3 areas of 1 cm2 per axon cross section and calculation of the neurofilament densities (number per μm2 of axon cross section devoid of other organelles).

  4. R. L. Friede andT. Samorajsky, Anat. Rec.167, 379 (1970).

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  5. R. Mayr, 66. Vers. Anat. Ges., Zagreb 1971, Anat. Anz., in press.

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Authors and Affiliations

  1. 2. Anatomisches Institut der Universität Wien, Währingerstrasse 13, A-1090, Wien, Austria

    W. Zenker, R. Mayr & H. Gruber

Authors
  1. W. Zenker
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  2. R. Mayr
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  3. H. Gruber
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Additional information

This investigation was supported by the ‘Fonds zur Förderung der wissenschaftlichen Forschung in Österreich’.

The authors gratefully acknowledge the excellent technical assistance of MissL. Henning and MissB. Fritsche.

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Zenker, W., Mayr, R. & Gruber, H. Axoplasmic organelles: Quantitative differences between ventral and dorsal root fibres of the rat. Experientia 29, 77–78 (1973). https://doi.org/10.1007/BF01913263

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