Planta

, Volume 57, Issue 2, pp 202–214 | Cite as

Die Auswirkungen von Mangan-Mangel auf Wachstum und Photosynthese bei der BlaualgeAnacystis Nidulans

  • Gerhard Richter
Article

Summary

  1. 1.

    The strict photoautotrophic blue-green alga,Anacystis nidulans, has a high requirement for manganese; its absence from the culture medium causes significant changes in the morphology and the metabolism of the cells.

     
  2. 2.

    These effects though comparable with those described for green algae are more severe and irreversible. After 5 days of culture in manganese deficient medium cell division is increasingly inhibited while cell growth continues giving rise to filamentous giant cells.

     
  3. 3.

    Net synthesis of chlorophyll ceases after 5 days, that of carotenoids after 10 days; phycocyanin and RNA are synthesized at a small rate up to 15 days.

     
  4. 4.

    Photosynthesis of deficient cells (10 days old) as measured by their oxygen production at high light intensities is reduced to 60% of the rate of normal cells when based upon their chlorophyll content; based upon equal cell volume the rate of the deficient cells was only 28% of the normal ones.

     
  5. 5.

    After short-time photosynthesis with radioactive bicarbonate the14C-fixation in the petrolether, the alcohol-water and the insoluble fraction from deficient cells was significantly lower as compared with the corresponding fractions from normal cells.

     
  6. 6.

    The alcohol-water fraction from manganese deficient cells differs in the distribution pattern of the incorporated14C from that of normal cells insofar as aspartic acid has a higher, glutamic acid a lower content of14C. Moreover, no labelling of glycolic acid, malic acid and alanine occurs in deficient cells during these short-time incorporation experiments.

     
  7. 7.

    The results indicate that a correlation exists between high manganese requirement and strict photoautotrophy; inAnacystis the relation of manganese to photosynthesis is obviously not restricted to the mechanism of oxygen liberation but seems to exist on the reductive side of photosynthesis, too.

     

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Copyright information

© Springer-Verlag 1961

Authors and Affiliations

  • Gerhard Richter
    • 1
  1. 1.Botanischen Institut der Universität TübingenTübingen

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