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Decrease in ventricular beta-adrenergic receptors in trained diabetic rats

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Summary

The effects of physical training on beta-adrenergic receptors were evaluated in heart ventricular tissue of diabetic rats. Mild diabetes mellitus was induced in rats with streptozotocin (45 mg/kg, iv). They were then submitted to a progressive 10-week running programme on a treadmill. Binding studies were done at six different concentrations of (−) [3H] dihydroalprenolol (0.5 to 14.4 nM) with ventricular membrane preparations from control (n=13), sedentary diabetic (n=9) and trained diabetic rats (n=10). Direct linear plot analysis of the data revealed that the total number of beta-adrenoceptors was reduced in sedentary diabetic rats as compared to control (2231±207 vs 2922±211 fmol/ventricules; P<0.05); however, there was no significant change in the receptor density expressed as fmol/mg of membrane protein (40±3 vs 43±3; P>0.05). On the other hand, the beta-adrenergic binding sites were decreased in trained diabetic rats, either expressed as the total number of receptors (1920±179 vs 2922±211; P<0.01), or as fmol/mg of membrane protein (30±3 vs 43±3; P<0.01). There was no significant change in the dissociation constant (KD) of these receptors between groups (KD=4.08±0.51, 4.69±0.93 and 2.88±0.39 nM respectively for control, sedentary diabetic and diabetic trained animals). The basal epinephrine concentration was significantly increased in trained diabetic rats (102±21 pg/ml vs 47±7 for control (P<0.05) and vs 49±9 for sedentary diabetic (P<0.05). These data indicate that a programme of physical training in diabetic rats can reduce both the total number and the density of beta-adrenoceptors in heart ventricular tissue, possibly by increasing the basal epinephrine secretion.

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  1. A. Nadeau M.D., F.R.C.P. (C)

    Present address: Laboratoires de Recherches en Endocrinologie et Métabolisme, Le Centre Hospitalier de l'Université Laval, 2705, Boul. Laurier, G1V 4G2, Québec, Québec, (Canada)

Authors and Affiliations

  1. Laboratoires de Recherches en Endocrinologie et Métabolisme, Le Centre Hospitalier de l'Université Laval, 2705, Boul. Laurier, G1V 4G2, Québec, Québec, (Canada)

    L. Sylvestre-Gervais, G. Tancrède, Minh-Hau Nuyen & S. Rousseau-Migneron

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  1. L. Sylvestre-Gervais
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  3. G. Tancrède
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  5. S. Rousseau-Migneron
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Sylvestre-Gervais, L., Nadeau, A., Tancrède, G. et al. Decrease in ventricular beta-adrenergic receptors in trained diabetic rats. Basic Res Cardiol 79, 432–439 (1984). https://doi.org/10.1007/BF01908143

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  • DOI: https://doi.org/10.1007/BF01908143

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