Basic Research in Cardiology

, Volume 77, Issue 1, pp 82–92 | Cite as

Metabolic changes in modified smooth muscle cells of rabbit carotid arteries

  • H. Heinle
  • F. Stowasser
  • E. Betz
Original Contributions

Summary

In the common carotid artery of rabbits, intimal myocyte proliferations were induced by daily repeated local electrical stimulation of the vessel wall in combination with a cholesterol containing diet given for 4 weeks. Some biochemical parameters of the morphologically modified intimal smooth muscle cells were studied and compared with those of samples obtained from nonstimulated tunica media of the contralateral carotid artery. The results show that in relation to dry weight both alkali extractable protein and DNA content of the proliferates are increased to about 125%. In the proliferates, the in-vivo tissue concentrations of glucose and glycogen are only 50–70% of the normal values, whereas the concentration of lactate is increased to about 160%. In-vitro incubation experiments of excised tissue samples from the intimal proliferates and normal media indicate that under an optimal supply of substrates the glucose uptake and lactate production of the proliferates are increased to 140% and 150%, respectively. This result provides evidence for an increased capacity of glycolysis in the proliferates, which in vivo may lead to a decrease in glucose concentration and to an increased concentration of lactate. This investigation shows that modified smooth muscle cells proliferating in the arterial intima exhibit an activated metabolism as seen in other models of arteriosclerosis.

Key words

model of arteriosclerosis electrical stimulation vascular smooth muscle cell proliferation modified myocytes glucose metabolism 

Zusammenfassung

In der Arteria carotis communis von Kaninchen wurden durch wiederholte, lokale elektrische Reizung intimale Myozytenproliferate hervorgerufen. Einige Stoffwechselparameter der morphologisch modifizierten Intimamyozyten wurden verglichen mit denen glatter Muskelzellen, die aus der Media unstimulierter Gefäßabschnitte stammten. Die Ergebnisse zeigen, daß der Gehalt an DNA und alkaliextrahierbarem Protein in den Proliferaten um etwa 25% erhöht ist. Ebenso ist der laktatspiegel erhöht (etwa um 60%), während die Konzentrationen von Glukose und Glykogen auf etwa 50–70% der Normalwerte absinken. In-vitro-Inkubationen von Gewebeproben aus intimalen Proliferaten und normaler Media zeigen, daß die Glukoseaufnahme und die Laktatproduktion um 40 bzw. 50% gesteigert sind. Diese Ergebnisse lassen den Schluß zu, daß die Zellen des Proliferates eine erhöhte Glykolysekapazität aufweisen, die unter In-vivo-Verhältnissen zu einer verminderten Glukose- und Glykogenkonzentration und zu einem erhöhten Laktatgehalt im Gewebe führt. Das heißt, daß in Übereinstimmung mit anderen Arteriosklerosemodellen sich die morphologisch modifizierten Intimamyozyten auch durch einen gesteigerten Stoffwechsel von den normalen Mediamyozyten unterscheiden.

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Copyright information

© Dr. Dietrich Steinkopff Verlag 1982

Authors and Affiliations

  • H. Heinle
    • 1
  • F. Stowasser
    • 1
  • E. Betz
    • 1
  1. 1.Physiologisches Institut (I) der Universität TübingenTübingen

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