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Pflügers Archiv - European Journal of Physiology

, Volume 412, Issue 4, pp 427–433 | Cite as

Effect of acute metabolic acidosis on transmembrane electrolyte gradients in individual renal tubule cells

  • F. -X. Beck
  • M. Schramm
  • A. Dörge
  • R. Rick
  • K. Thurau
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

We studied the effect of acute metabolic acidosis on potassium, sodium and chloride gradients across the apical membrane of proximal and distal tubule cells by determining electrolyte concentrations in individual cells and in tubule fluid employing electron microprobe analysis. Cellular measurements were performed on freeze-dried cryosections of the renal cortex, analysis of tubule fluid electrolyte concentrations on freeze-dried microdroplets of micropuncture samples obtained from proximal and from early and late distal collection sites. Acidosis (NH4Cl i.v. and i.g.) induced a substantial rise in plasma potassium concentration without significant effects on cell potassium concentrations. Potassium concentrations along the surface distal tubule were also unaltered; thus the chemical driving force for potassium exit from cell to lumen was not affected by acidosis. In all but intercalated cells acidosis markedly increased cell phosphorus concentration and cell dry weight indicating cell shrinkage and thus diminution of cell potassium content. Because the increase in intracellular chloride concentration exceeded the increase in plasma chloride concentration, the chemical chloride gradient across the contraluminal membrane was markedly depressed by acidosis.

Key words

Acute metabolic acidosis Renal distal electrolyte transport Renal cell electrolyte concentrations Individual distal tubule cells Transmembrane electrolyte concentration gradients Electron microprobe analysis 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • F. -X. Beck
    • 1
  • M. Schramm
    • 1
  • A. Dörge
    • 1
  • R. Rick
    • 2
  • K. Thurau
    • 1
  1. 1.Physiologisches Institut der Universität MünchenMünchen 2Federal Republic of Germany
  2. 2.Medical School, Department of Physiology and BiophysicsUniversity of Alabama in BirminghamBirminghamUSA

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