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Positive inotropic and chronotropic effects of the calcium ionophore A23187 (calimycin) on guinea-pig atria

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Summary

The inotropic and chronotropic effects of the calcium ionophore A23187 (calimycin=CA) in isolated, superfused, electrically driven, auxotonically contracting left and spontaneously beating right guinea-pig atria were examined at different Ca2+ concentrations. 10−6 to 10−5 mol/l CA shows significantly positive inotropic and chronotropic effects. The inotropic effect of CA can be significantly diminished by relatively high concentrations of Ca2+ channel antagonists and relatively low concentrations of ryanodine. The positive chronotropic effect of CA can be reduced slightly, but significantly, by beta-adrenoceptor antagonists and histamine H2-receptor antagonists. From this we infer that the positive inotropic effect of CA is mainly due to the release of Ca2+ from cardiac sarcoplasmic reticulum, whereas catecholamine and histamine release appear to contribute to the positive chronotropic effect of the ionophore.

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Author notes

  1. H. M. Himmel

    Present address: Pharmakologisches Institut, Universitätsklinikum Essen, Hufelandstr. 55, 4300, Essen 1, FRG

Authors and Affiliations

  1. Department of Pharmacology, University of Essen, Essen, FRG

    H. M. Himmel & M. Siess

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  1. H. M. Himmel
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  2. M. Siess
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Additional information

Preliminary results were presented at the 28th Spring Meeting of the “Deutsche Gesellschaft für Pharmakologie und Toxikologie”, March 10–13, 1987, Mainz, and at the “Herbsttagung der Deutschen Gesellschaft für Herz- und Kreislaufforschung”, October 8–10, 1987, Freiburg.

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Himmel, H.M., Siess, M. Positive inotropic and chronotropic effects of the calcium ionophore A23187 (calimycin) on guinea-pig atria. Basic Res Cardiol 83, 167–175 (1988). https://doi.org/10.1007/BF01907271

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