Summary
Radioactively labeled microspheres were used to investigate the effect of 2′,3′-di-O-nitro-5′-(N-ehtylcarboxamido)-adenosine (10 μg/kg orally), a long-acting adenosine analog, on blood flow through various organs over an experimental period of three hours in conscious dogs. There was a significant increase in myocardial, cerebral, intestinal, and renal cortical blood flow, whereas the flow through skeletal muscle, liver, pancreas, and renal medulla was unaffected. The endocardial/epicardial flow ratio, which was more than one under control conditions, decreased significantly. Arterial blood pressure and heart rate remained virtually unchanged. Since the adenosine analog is rapidly and completely denitratedin vivo, the denitration product 5′-(N-ethylcarboxamide)-adenosine, a highly potent adenosine receptor agonist, must be considered as the vasoactive metabolite.
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References
Cobb, F. R., R. J. Bache, J. C. Greenfield, Jr.: Regional myocardial blood flow in awake dogs. J. Clin. Invest.53, 1618–1625 (1974).
Drury, A. N., A. Szent-György: The physiological activity of adenosine compounds with their especial reference to their action upon the mammalian heart. J. Physiol. (London)68, 213–237 (1929).
Haber, E., T. Körner, L. B. Page, B. Kliman, A. Purnode: Application of a radioimmunoassay for angiotensin I to the physiologic measurement of plasma renin activity in normal human subjects. J. Clin. Endocrinol. Metab.29, 1349–1355 (1969).
Haddy, F. J., J. B. Scott: Metabolically linked vasoactive chemicals in local regulation of blood flow. Physiol. Rev.48, 688–707 (1968).
Hashimoto, K., S. Kumakura: The pharmacological features of the coronary, renal, mesenteric and femoral arteries. Jap. J. Physiol.15, 510–551 (1965).
Osswald, H., W. S. Spielman, F. G. Knox: Mechanism of adenosine-induced decreases in glomerular filtration rate in dogs. Circulat. Res.43, 465–470 (1978).
Raberger, G. W. Schütz, O. Kraupp: Coronary dilatory action of adenosine analogues: a comparative study. Arch. Int. Pharmacodyn. Ther.230, 140–149 (1977).
Raberger, G., W. Schütz, O. Kraupp: Cardiovascular and metabolic actions of an adenosine analogue (2′,3′-O-methoxyethylidene-adenosine-5′-ethylcarboxamide) in the conscious dog. Arch. Int. Pharmacodyn. Ther.232, 150–157 (1978).
Schöndorf, H., W. Rummel, K. Pfleger: dosis-Wirkungs-Beziehung zwischen Koronardurchfluß und Herzfrequenz für Adenosin, Experientia25, 44 (1965).
Schütz, W., G. Raberger, O. Kraupp: Evidence for glucagon-releasing activity of vasoactive adenosine analogs in the conscious dog, Naunyn-Schmiedeberg's Arch. Pharmacol.304, 249–254 (1978).
Schütz, W., G. Raberger, O. Kraupp: Influence of aminophylline or β-adrenoceptor blockade on glucagon release by a vasoactive adenosine analogue. Arch. Int. Pharmacodyn. Ther.234, 214–220 (1978).
Schütz, W., G. Steurer, E. Tuisl: Functional identification of adenylate cyclase-coupled adenosine receptors in rat brain microvessels. Eur. J. Pharmacol.85, 177–184 (1982).
Spielman, W. S., S. L. Britton, M. J. Fiksen-Olsen: Effect of adenosine on the distribution of renal blood flow in dogs. Circulat. Res.46, 449–456 (1980).
Wiener, H., P. Krivanek, N. Kolassa: Metabolism and disposition of 2′,3′-di-O-nitro-adenosine-5′-(N-ethyl-carboxamide) in dogs. Biochem. Pharmacol., in press.
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Schütz, W., Kraupp, O., Bacher, S. et al. The effect of a long-acting adenosine analog on blood flow through various organs in the dog. Basic Res Cardiol 78, 679–684 (1983). https://doi.org/10.1007/BF01907215
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DOI: https://doi.org/10.1007/BF01907215