Summary
Localized elemental concentrations of Ca, P, Cl, S, and Zn were detected by X-ray microanalysis during viewing of ultrathin myocardial sections. Cardiomyopathic hamsters were compared with a healthy strain of the same age. Significant increases in calcium and chlorine concentrations were found in sick animals. These were highest in the hypertrophic stage of the disease. Phosphorus and zinc were more variable. Ratios between elements (especially P/Ca) in the same cells and of the same elements in different cell types were found to offer numerical criteria for estimating the degree of pathological change.
Zusammenfassung
Die lokale Konzentration von Ca, P, Cl, S und Zn in ultradünnen Myokardschnitten wurden durch Röntgenmikroanalyse untersucht. Hamster mit Kardiomyopathie wurden mit einem gesunden Stamm gleichen Alters verglichen. Es wurde ein signifikanter Anstieg der Ca- und Cl-Konzentration bei den kranken Tieren gefunden, am ausgeprägtesten im hypertrophischen Stadium der Erkrankung. Die Konzentrationen von P und Zn waren unterschiedlicher. Es ergab sich, daß das Verhältnis zwischen den Elementen (besonders P/Ca) innerhalb der gleichen Zelle und das Verhältnis der gleichen Elemente innerhalb verschiedener Zelltypen quantitative Kriterien für die Bestimmung des Ausmaßes pathologischer Veränderungen bietet.
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With 2 figures and 3 tables
This work was supported by the: British Heart Foundation, British Science Research Council and the Hebrew University-Hadassah Medical School.
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Yarom, R., Hall, T.A. & Oakley, C.M. Localised concentrations of elements in hamster cardiomyopathy. Basic Res Cardiol 72, 660–670 (1977). https://doi.org/10.1007/BF01907046
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DOI: https://doi.org/10.1007/BF01907046