Skip to main content
SpringerLink
Log in

Atrial and ventricular myosins from human hearts II. Isoenzyme distribution after myocardial infarction

  • Original Contributions
  • Published:
Basic Research in Cardiology Aims and scope Submit manuscript

Summary

Human atrial and ventricular myosins were prepared from autopsy specimens from subjects with coronary heart disease. Cardiac myosin light chain isotypes were resolved using twodimensional gel electrophoresis, whereas myosin isozymes were detected by pyrophosphate gel electrophoresis.

Myocardial infarction and associated work overload cause a transition in the light chain complements of the myosins. Thus ventricular myosin light chains were found in pressure overloaded atria and atrial light chains have also been identified in the infarct ventricle of the human heart.

Two molecular isoenzymes of the human atrial myosin, the relative proportions of which are changed after infarction, were separated under non-dissociating conditions by gel electrophoresis. A decrease in HA-3 and a corresponding increase in HA-1 were observed. Ventricular hypertrophy in patients with coronary insufficiency induces a second ventricle isomyosin, called HV-1, with the same electrophoretic mobility as HA-1. The relative part of this myosin type amounts to 20%. Comparative peptide mapping studies were carried out on myosin subfragment-1 preparations from normal and infarct ventricles. In the primary structures, the chymotrypsic digestions produced slight differences.

These data demonstrate the heterogeneity of human atrial and ventricular myosins in patients with coronary heart disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Banerjee SK, Wiener J (1983) Effects of aging on atrial and ventricular human myosins. Basic Res Cardiol 78:685–694

    PubMed  Google Scholar 

  2. Bouvagnet P, Leger J, Pons F, Dechesne CA, Leger JJ (1984) Fiber types and myosin types in human atrium and ventricular myocardium, Circ Res 55:794–804

    PubMed  Google Scholar 

  3. Bouvagnet P, Leger J, Dechesnc CA, Dureau G, Anoal M (1985) Local changes in myosin types in disease human atrial myocardium: a quantitative immunofluorescence study. Circulation 72:272–279

    PubMed  Google Scholar 

  4. Catanzaro DF, O'Connell AM, Morris BJ (1985) Isolation of genomic clones for the heavy chains of two human cardiac myosins. Clin Exper Pharmacol Physiol 12:295–297

    Google Scholar 

  5. Chizzonite RA, Zak R (1984) Regulation of myosin isoenzyme composition in fetal and neonatal rat ventricle by endogenous thyroid hormones. J Biol Chem 259:12 628–12 632

    Google Scholar 

  6. Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK (1977) Peptide mapping by limited proteolysis in sodium dedecyl sulfate and analysis by gel electrophoresis. J Biol Chem 252:1102–1106

    PubMed  Google Scholar 

  7. Cummins P (1983) Primitive nature of atrial muscle is reflected in human myosin light chain composition and response hypertrophy. In: Alpert NR (ed) Perspectives in Cardiovascular Research. Myocardial hypertrophy and failure. Vol 7, Raven Press, New York, pp 417–423

    Google Scholar 

  8. Cummins P (1982) Transitions in human atrial and ventricular myosin light chain isoenzymes in response to cardiac-pressure overload-induced hypertrophy. Biochem J 205:195–204

    PubMed  Google Scholar 

  9. Dechesne C, Leger J, Bouvagnet P, Claviez M, Leger JJ (1985) Fractionation and characterization of two molecular variants of myosin from adult human atrium. J Mol Cell Cardiol 17:753–767

    PubMed  Google Scholar 

  10. Fetisova TV, Zabolotnyuk IP (1965) Changes in heart muscle and liver proteins in experimental myocardial infarction. Fed Proc 24:T 960-T 962

    Google Scholar 

  11. Gorza L, Mercadier JJ, Schwarz K, Thornell LE, Sartore S, Schiaffino S (1984) Myosin types in the human heart. An immunofluorescence study of normal and hypertrophied atrial and ventricular myocardium. Circ Res 54:694–702

    PubMed  Google Scholar 

  12. Gorza L, Sartore S, Schiaffino S (1982) Myosin types and fiber types in cardiac muscle II. Atrial myocardium. J Cell Biol 95:838–845

    PubMed  Google Scholar 

  13. Gulian GG, Moss RL, Greaser M (1983) Improved methodology for analysis and quantification of proteins on one-dimensional silver stained slab gels. Anal Biochem 129:277–289

    PubMed  Google Scholar 

  14. Hirzel HO, Tuchschmid CR, Schneider J, Krayenbuehl HP, Schaub MC (1985) Relationship between myosin isoenzyme composition, hemodynamics, and myocardial structure in various forms of human cardiac hypertrophy. Circ Res 57:729–740

    PubMed  Google Scholar 

  15. Hoh JFY, McGroth MA, Hale PT (1978) Electrophoretic analysis of multiple forms of rat cardiac myosins: effects of hypophysectomy and thyroxime replacement. J Mol Cell Cardiol 10:1053–1076

    PubMed  Google Scholar 

  16. Hoffmann U, Axmann C, Grisk A (1986) Myosin isoenzymes in normal and hypertrophied human hearts. Biochim Biomed Acta 43:951–962

    Google Scholar 

  17. Hoffmann U, Siegert E (1987) Atrial and ventricular myosins from human hearts. I. Isoenzyme distribution during development and in the adult. Basic Res Cardiol 82:348–358

    PubMed  Google Scholar 

  18. Klotz C, Swynghedauw B, Mendes H, Marotte F, Leger JJ (1981) Evidence for new forms of cardiac myosin heavy chains in mechanical heart overloading and in aging. Eur J Biochem 115:415–421

    PubMed  Google Scholar 

  19. Klotz C, Auimont MC, Leger JJ, Swynghedauw B (1975) Human cardiac myosin ATPase and light subunits. A comparative study, Biochim Biophys Acta 386:461–469

    PubMed  Google Scholar 

  20. Kozlovskis PL, Silver JD, Rubin RW, Wong SS, Gaide MS, Bassett AL, Cameron JS, Myerburg RJ (1984) Regional variations in myosin heavy chain concentration after healing of experimental myocardial infarction in cats. J Mol Cell Cardiol 16:559–566

    PubMed  Google Scholar 

  21. Kuro-O M, Tschuchimochi H, Ueda S, Takaku F, Yazaki Y (1986) Distribution of cardiac myosin isoenzymes in human conduction system. Immunohistochemical study using monoclonal antibodies. J Clin Invest 77:340–347

    PubMed  Google Scholar 

  22. Leclerc JF, Swynghedauw B (1976) Myofibrillar ATPase, DNA, and hydroxyproline content of human hypertrophied heart. Eur J Clin Invest 6:27–33

    PubMed  Google Scholar 

  23. Leger JOC, Bouvagnet P, Pau B, Roncucci R, Leger JJ (1985) Levels of ventricular myosin fragments in human sera after myocardial infarction, determined with monoclonal antibodies to myosin heavy chains. Eur J Clin Invest 15:422–429

    PubMed  Google Scholar 

  24. Lompre AM, Mercadier JJ, Wisnewsky C, Bouveret P, Pantaloni C, D'Albis A, Schwartz K (1981) Species- and age-dependent changes in the relative amounts of cardiac myosin isoenzymes in mammals. Dev Biol 84:286–290

    Google Scholar 

  25. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  Google Scholar 

  26. Lukashevich E, Ivanova AG (1976) Effect of acid cathepsins on myosin and sarcoplasmic proteins of normal and denervated rat muscles. Ukr Biokhim Zh 75:555–558

    Google Scholar 

  27. Mahdavi V, Chambers AP, Nadal-Ginard B (1984) Cardiac α- and β-myosin heavy chain genes are organized in tandem. Proc Natl Acad Sci USA 81:2626–2630

    PubMed  Google Scholar 

  28. Malhotra A, Bhan A, Scheuer J (1977) Biochemical characteristics of human cardiac myosin. J Mol Cell Cardiol 9:73–80

    PubMed  Google Scholar 

  29. Mercadier JJ, Bouveret P, Gorza L, Schiaffino S, Clark WA, Zak R, Swynghedauw B, Schwartz K (1983) Myosin isoenzymes in normal and hypertrophied human ventricular myocardium. Circ Res 53:52–62

    PubMed  Google Scholar 

  30. Mercadier JJ, Lompre AM, Bouveret P, Rapporort L, Swynghedauw B, Schwartz K (1983) Myosin isoenzymic distribution in hypertrophied rat and human hearts. In: Jacob R, Gulch RW, Kissling G (eds) Cardiac adaptation to hemodynamic overload, training and stress. Steinkopff Darmstadt, pp 104–112

    Google Scholar 

  31. Nagai R, Chiu CC, Yamaoki K, Ohuchi Y, Ueda S, Imataka K, Yazaki Y (1983) Evaluation of methods for estimating infarct size by myosin LC-2: comparison with cardiac enzymes. Am J Physiol 245:H413-H419

    PubMed  Google Scholar 

  32. O'Farrell PH (1975) High resolution two dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

    PubMed  Google Scholar 

  33. Pagani ED, Solaro RJ (1983) Swimming exercise, thyroid state, and the distribution of myosin isoenzymes in rat heart. Am J Physiol 245:H 713-H 720

    Google Scholar 

  34. Poole-Wilson PA (1978) Measurement of myocardial intracellular pH in pathological states. J Mol Cell Cardiol 10:511–526

    PubMed  Google Scholar 

  35. Price KM, Littler WA, Cummins P (1980) Human atrial and ventricular myosin light-chain subunits in the adult during development. Biochem J 191:571–580

    PubMed  Google Scholar 

  36. Prince HP, Trayer HR, Henry GD, Trayer IP, Dalgarno DC, Levine BA, Cary PD, Turner C (1981) Proton nuclear-magnetic resonance spectroscopy of myosin subfragment-1 isoenzymes. Eur J Biochem 121:213–219

    PubMed  Google Scholar 

  37. Regan TJ, Effros RM, Haider B, Oldewurtel HA, Ettinger PO, Ahmed SS (1976) Myocardial ischemia and cell acidosis: modification by alkali and the effects on ventricular function and cation composition. Am J Cardiol 37:501–507

    PubMed  Google Scholar 

  38. Rupp H, Popova N, Jacob R (1983) Dissociation between factors in hypertrophy and changes in myosin isoenzymes population of the pressure-loaded rat heart. In: Jacob R, Gulch RW, Kissling G (eds) Cardiac adaptation to hemodynamic overload, training and stress. Steinkopff, Darmstadt, pp 46–52

    Google Scholar 

  39. Sartore S, Gorza L, Pierobon Bormioli S, Dalla Libera L, Schiaffino S (1981) Myosin types and fiber types in cardiac muscle. I. Ventricular myocardium. J Cell Biol 88:226–233

    PubMed  Google Scholar 

  40. Schaub MC, Tuchschmid CR, Srihari T, Hirzel HO (1984) Myosin isoenzymes in human hypertrophic hearts. Shift in atrial myosin heavy chains and in ventricular myosin light chains. Eur Heart J (Suppl F) 5:85–93

    Google Scholar 

  41. Schiaffino S, Gorza L, Saggin L, Valfre C, Sartore S (1984) Myosin changes in hypertrophied human atrial and ventricular myocardium. A correlated immunofluorescence and quantitative immunochemical study on serial cryosections. Eur Heart J (Suppl F) 5:95–102

    Google Scholar 

  42. Schier JJ, Adelstein RS (1982) Structural and enzymatic comparison of human cardiac muscle myosin isolated from infants, adults, and patients with hypertrophic cardiomyopathy. J Clin Invest 69:816–825

    PubMed  Google Scholar 

  43. Schwartz WN, Bird JWC (1977) Cathepsins B and D: purification and degradation of myofibrillar proteins. Biochem J 167:811–815

    PubMed  Google Scholar 

  44. Shlesinger H, Ovil Y, Levy MJ, Kessler-Icekson G (1985) Separation and quantitation of ventricular myosin heavy-chain from human atrial myocardium. Biochem Intern 11:747–753

    Google Scholar 

  45. Shiverick KT, Thomas LL, Alpert NR (1975) Purification of cardiac myosin. Biochim Biophys Acta 393:124–133

    PubMed  Google Scholar 

  46. Smitherman TC, Dycus DW, Richards EG (1980) Dissociation of myosin light chains and decreased myosin ATPase activity with acidification of synthetic myosin filaments: possible clues to the fate of myosin in myocardial ischemia and infarction. J Mol Cell Cardiol 12:149–164

    PubMed  Google Scholar 

  47. Smitherman TC, Richards EG (1980) Degradation and alteration of contractile proteins: myocardial ischemia and infarction. In: Wildenthal K (ed) Degradative process in heart and skeletal muscle. Elsevier, North-Holland Biomedical Press, pp 361–376

  48. Srihari T, Tuchschmid CR, Schaub MC (1982) Isoforms of heavy and light chains of cardiac myosins from rat and rabbit. Basic Res Cardiol 77:599–609

    PubMed  Google Scholar 

  49. Swynghedauw B, Schwartz K, Leger JJ (1977) Cardiac myosin. Phylogenic and pathologic changes. Basic Res Cardiol 72:254–260

    PubMed  Google Scholar 

  50. Takeda K, Dominiak P, Turck D, Rupp H, Jacob R (1985) The influence of endurance training on mechanical catecholamine responsiveness, β-adrenoceptor density and myosin isoenzyme pattern of rat ventricular myocardium. Basic Res Cardiol 80:88–99

    PubMed  Google Scholar 

  51. Tuchschmid CR, Srihari T, Hirzel HO, Schaub MC (1983) Structural variants of heavy and light chains of atrial and ventricular myosins in hypertrophied human hearts. In: Jacob R, Gülch RW, Kissling G (eds) Cardiac adaptation to hemodynamic overload, training and stress. Steinkopff, Darmstadt, pp 123–128

    Google Scholar 

  52. Whalen RG, Sell SM, Eriksson A, Thornell LE (1982) Myosin subunit types in skeletal and cardiac tissues and their developmental distribution. Develop Biol 91:478–484

    PubMed  Google Scholar 

  53. Weeds AG, Pope B (1977) Studies on the chymotryptic digestion of myosin effects of divalent cations on proteolytic susceptibility. J Mol Biol 111:129–157

    PubMed  Google Scholar 

  54. Yamamoto K, Sekine T (1983) Interaction of alkali light chain-1 with actin: effect of ionic strength on the cross-linking of alkali light chain-1 with actin. J Biochem 94:2075–2078

    PubMed  Google Scholar 

  55. Yazaki Y, Tsuchimochi H, Koro-O M, Kurabayashi M, Isobe M, Ueda S, Nagai R, Takaku F (1984) Distribution of myosin isozymes in human atrial and ventricular myocardium: comparison in normal and overloaded heart. Eur Heart J (Suppl F) 5:103–110

    Google Scholar 

Download references

Author information

Author notes

  1. U. Hoffmann

    Present address: Institut für Pharmakologie und Toxikologie, Ernst-Moritz-Arndt-Universität, Friedrich-Loeffler-Str. 23d, 2200, Greifswald, DDR

Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie, Ernst-Moritz-Arndt-Universität, Friedrich-Loeffler-Str. 23d, 2200, Greifswald, DDR

    C. Axmann & N. Palm

Authors
  1. U. Hoffmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Axmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. Palm
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hoffmann, U., Axmann, C. & Palm, N. Atrial and ventricular myosins from human hearts II. Isoenzyme distribution after myocardial infarction. Basic Res Cardiol 82, 359–369 (1987). https://doi.org/10.1007/BF01907023

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01907023

Key words

Search

Navigation