Effect of sulfhydryl group modification on age-associated alteration of actomyosin ATPase activity in human myocardium
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Cardiac ventricular actomyosin was prepared from autopsy samples from humans ranging in age from one to 83 years, and its Ca2+-ATPase and K+-EDTA-ATPase activities were determined in the presence or absence of a sulfhydryl reagnet, N-ethylmaleimide (NEM). The Ca2+-ATPase activity increased in the presence of appropriate concentrations of NEM. The extent of the stimulation of Ca2+-ATPase activity by NEM decreased significantly with age. The ratio of K+-EDTA-ATPase activity to Ca2+-ATPase activity also decreased with age. This suggests that there is an age-related modification of sulfhydryl groups in the myosin molecule.
Key wordsage myocardium actomyosin ATPase activity sulfhydryl groups
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