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Basic Research in Cardiology

, Volume 85, Issue 1, pp 2–8 | Cite as

Effect of sulfhydryl group modification on age-associated alteration of actomyosin ATPase activity in human myocardium

  • K. Yoshida
  • R. Matoba
  • N. Fujitani
  • S. Onishi
Original Contributions

Summary

Cardiac ventricular actomyosin was prepared from autopsy samples from humans ranging in age from one to 83 years, and its Ca2+-ATPase and K+-EDTA-ATPase activities were determined in the presence or absence of a sulfhydryl reagnet, N-ethylmaleimide (NEM). The Ca2+-ATPase activity increased in the presence of appropriate concentrations of NEM. The extent of the stimulation of Ca2+-ATPase activity by NEM decreased significantly with age. The ratio of K+-EDTA-ATPase activity to Ca2+-ATPase activity also decreased with age. This suggests that there is an age-related modification of sulfhydryl groups in the myosin molecule.

Key words

agmyocardium actomyosin ATPase activity sulfhydryl groups 

Abbreviations

NEM

N-ethylmaleimide

EDTA

ethylenediamine-tetraacetic acid

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Copyright information

© Dr. Dietrich Steinkopff Verlag 1990

Authors and Affiliations

  • K. Yoshida
    • 1
  • R. Matoba
    • 1
  • N. Fujitani
    • 1
  • S. Onishi
    • 2
  1. 1.Department of Legal MedicineOsaka University Medical SchoolOsakaJapan
  2. 2.Department of Pathology, College of Biomedical TechnologyOsaka UniversityToyonaka-shi, OsakaJapan

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