Basic Research in Cardiology

, Volume 85, Issue 1, pp 2–8 | Cite as

Effect of sulfhydryl group modification on age-associated alteration of actomyosin ATPase activity in human myocardium

  • K. Yoshida
  • R. Matoba
  • N. Fujitani
  • S. Onishi
Original Contributions


Cardiac ventricular actomyosin was prepared from autopsy samples from humans ranging in age from one to 83 years, and its Ca2+-ATPase and K+-EDTA-ATPase activities were determined in the presence or absence of a sulfhydryl reagnet, N-ethylmaleimide (NEM). The Ca2+-ATPase activity increased in the presence of appropriate concentrations of NEM. The extent of the stimulation of Ca2+-ATPase activity by NEM decreased significantly with age. The ratio of K+-EDTA-ATPase activity to Ca2+-ATPase activity also decreased with age. This suggests that there is an age-related modification of sulfhydryl groups in the myosin molecule.

Key words

agmyocardium actomyosin ATPase activity sulfhydryl groups 





ethylenediamine-tetraacetic acid


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Alpert NR, Gordon MS (1962) Am J Physiol, 202:940–946PubMedGoogle Scholar
  2. 2.
    Banerjee SK, Wiener J (1983) Basic Res Cardiol 78:685–694PubMedGoogle Scholar
  3. 3.
    Bradford MM (1976) Anal Biochem 72:248–254PubMedGoogle Scholar
  4. 4.
    Cummins P, Lambert SJ (1986) Circ Res 58:846–858PubMedGoogle Scholar
  5. 5.
    Hirzel HO, Tuchschmid CR, Schneider J, Krayenbuehl HP, Schaub MC (1985) Circ Res 57:729–740PubMedGoogle Scholar
  6. 6.
    Hoffmann U, Axmann C, Palm N (1987) Basic Res Cardiol 82:359–369PubMedGoogle Scholar
  7. 7.
    Hoh JFY, McGrath PA, Hale PT (1978) J Mol Cell Cardiol 10:1051–1076Google Scholar
  8. 8.
    Horigome T, Yamashita T (1977) J Biochem 82:1085–1092PubMedGoogle Scholar
  9. 9.
    Klotz C, Aumont MC, Leger JJ, Swynghedauw B (1975) Biochim Biophys Acta 386:461–469PubMedGoogle Scholar
  10. 10.
    Lanzetta PA, Alavaltz LJ, Reinach PS, Candia OA (1979) Anal Biochem 100:95–97PubMedGoogle Scholar
  11. 11.
    Laemmli UK (1970) Nature 227:680–685PubMedGoogle Scholar
  12. 12.
    Malhotra A, Bhan A, Scheuer J (1976) J Mol Cell Cardiol9:73–80Google Scholar
  13. 13.
    Mercadier JJ, Bouveret P, Gorza L, Schiaffino S, Clark WA, Zak R, Swynghedauw B, Schwartz K (1983) Circ Res 53:52–62PubMedGoogle Scholar
  14. 14.
    Price KM, Litter WA, Cummins P (1980) Biochem J 191:571–580PubMedGoogle Scholar
  15. 15.
    Scheuer J, Bhan AK (1979) Circ Res 45: 1–12PubMedGoogle Scholar
  16. 16.
    Schier JJ, Adelstein RS (1982) J Clin Invest 69:816–825PubMedGoogle Scholar
  17. 17.
    Sekine T, Kielley WW (1964) Biochim Biophys Acta 81:336–345Google Scholar
  18. 18.
    Shiverick KT, Hamrell BB, Alpert NR (1976) J Mol Cell Cardiol 8:837–851PubMedGoogle Scholar
  19. 19.
    Yazaki Y, Raben MS (1975) Circ Res 36:208–215Google Scholar
  20. 20.
    Yazaki Y, Ueda S, Nagai R, Shimada K (1979) Circ Res 45:522–527PubMedGoogle Scholar
  21. 21.
    Yazaki Y, Tsuchimochi H, Kuro-o M, Kurabayashi M, Isobe M, Ueda S, Nagai R, Takaku F (1984) Eur Heart J 5 (Suppl. F):103–110Google Scholar
  22. 22.
    Yoshida K, Kimura H (1986) Biochim Biophys Acta 880:197–203PubMedGoogle Scholar
  23. 23.
    Yoshida K, Fujitani T, Matoba R, Shikata S (1988) Jpn J Legal Med 42:358–362Google Scholar
  24. 24.
    Yoshida K, Matoba R, Fujitani N, Wakasugi C, Onishi S (1989) Age 12: 97–102Google Scholar

Copyright information

© Dr. Dietrich Steinkopff Verlag 1990

Authors and Affiliations

  • K. Yoshida
    • 1
  • R. Matoba
    • 1
  • N. Fujitani
    • 1
  • S. Onishi
    • 2
  1. 1.Department of Legal MedicineOsaka University Medical SchoolOsakaJapan
  2. 2.Department of Pathology, College of Biomedical TechnologyOsaka UniversityToyonaka-shi, OsakaJapan

Personalised recommendations