Skip to main content
SpringerLink
Log in

Age-dependent production of mitochondrial hydrogen peroxide, lipid peroxides and fluorescent pigments in the rat heart

  • Original Contributions
  • Published:
Basic Research in Cardiology Aims and scope Submit manuscript

Summary

Mitochondria were prepared from hearts of 3-, 14-, 18-, and 24-month-old male Wistar rats. Respiratory control ratio (RCR) values did not change with age in the glutamate or succinate-induced respiration except at 24 months in which RCR values significantly increased with both the substrates. Using still glutamate or succinate as substrates the production of H2O2 was measured in the presence of antimycin. A 70% and 25% increase in H2O2 formation was observed at 14 and 18 months of age, respectively, in comparison to the youngest group. Only in the presence of succinate was a 25% elevation in H2O2 found at 24 months of age. These observations parallel with the decrease of the ratio between tissue levels of reduced and oxidized glutathione that was observed at 14 and 18 months of age. The concentration of myocardial malondialdehyde, a secondary product of lipid peroxidation, remained the same at all ages measured, most probably because it is readly metabolized in vivo. On the contrary the myocardial level of lipofuscin, which is not degraded by the cell, progressively increased beginning from 18 months of age.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Awadi AB, Chattopadhyay JP (1983) Developmental alteration in 5-nucleotidase kinetics and lipid composition of rat heart sarcolemma. Mech Ageing Dev 22:151–158

    PubMed  Google Scholar 

  2. Bidlack WR, Tappel AL (1973) Damage to microsomal membrane by lipid peroxidation. Lipids 8:177–182

    PubMed  Google Scholar 

  3. Bradford MA (1976) A rapid and sensitive method of the quantitation of microgram quantities of protein utilizing the principle of protein dye binging. Anal Biochem 72:248–254

    PubMed  Google Scholar 

  4. Cadenas E, Boveris A (1980) Enhancement of hydrogen peroxide formation by protophores and ionophores in antimycin-supplemented mitochondria. Biochem J 188:31–35

    PubMed  Google Scholar 

  5. Canedas A, Boveris A, Ragan CI, Stoppani AOM (1977) Production of superoxide radicals, and hydrogen peroxide by NADH-ubiquinone reductase and ubiquinol-cytochrome c reductase from beef-heart mitochondria. Arch Biochem Biophys 180:248–257

    PubMed  Google Scholar 

  6. McCord JM (1985) Oxygen-derived free radicals in postischemia tissue injury. N Engl J Med 312:159–163

    PubMed  Google Scholar 

  7. Dillard CJ, Tappel AL (1984) Fluorescent damage products of lipid peroxidation. In: Packer L (ed) Method in enzymology, Vol 105. Academic Press Inc, London, pp 337–341

    Google Scholar 

  8. Floyd RA (1984) Free radicals in arylamine carcinogenesis. In: Pryor WA (ed) Free radicals in biology, Vol IV. Academic Press, New York, pp 187–208

    Google Scholar 

  9. Fridovich I (1978) The biology of free radicals. Science 201:875–880

    PubMed  Google Scholar 

  10. Guarnieri C, Flamigni F, Rossoni Caldarera C (1979) Glutathione peroxidase activity and release of glutathione from oxygen-deficient perfused rat heart. Biochem Biophys Res Commun 89:678–684

    PubMed  Google Scholar 

  11. Hassan HM, Fridovich I (1977) Regulation of the synthesis of superoxide dismutase in Escherichia coli. J Biol Chem 252:7667–7672

    PubMed  Google Scholar 

  12. Hazelton GA, Lang CA (1980) Glutathione contents of tissues in the aging mouse. Biochem J 188:25–30

    PubMed  Google Scholar 

  13. Jackson MJ, Jones DA, Edwards RHT (1983) Lipid peroxidation of skeletal muscle: an in vitro study. Bioscience Rep 3:609–619

    Google Scholar 

  14. Khanna VP, Yaneja SK, Raj HG (1982) Polyamines modify paraquat-induced changes in pulmonary superoxide dismutase and lipid peroxidation. Res Commun Chem Phatol Pharmacol 35:337–340

    Google Scholar 

  15. Lammi-Keefe CJ, Swan PB, Hegarty PVJ (1984) Copperzinc and manganese superoxide dismutase activities in cardiac and skeletal muscles during aging in male rats. Gerontology 30:153–158

    PubMed  Google Scholar 

  16. Leibowith BE, Siegel BV (1980) Aspects of free radicals reactions in biological systems. Aging J Gerontol 95:45–56

    Google Scholar 

  17. Lewin MB, Timiras PS (1984) Lipid change with aging in cardiac mitochondrial membranes. Mech Ageing Dev 24:343–351

    PubMed  Google Scholar 

  18. Loschen G, Azzi A (1976) On the formation of hydrogen peroxide and oxygen radicals in heart mitochondria. In: Harris P, Bing RJ, Fleckenstein A (eds) Biochemistry and pharmacology of myocardial hypertrophy, hypoxia and infarction; University Park Press, Baltimore. Vol 7, pp 3–12

    Google Scholar 

  19. Loschen G, Flohe L, Change B (1971) Respiratory chain-linked H2O2 production in pigeon heart mitochondria. FEBS Lett 18:261–264

    PubMed  Google Scholar 

  20. Makinodan T, Kay MB (1980) Age influence of the immune system. Adv Immunol 29:287–330

    PubMed  Google Scholar 

  21. Myers LS (1973) Free radical damage of nucleic acids and their components by ionizing radiation. Fed Proc 32:1882–1894

    PubMed  Google Scholar 

  22. Nohl H, Breuninger V, Hegner D (1978) Influence of mitochondrial radical formation on energy-linked respiration. Eur J Biochem 90:385–390

    PubMed  Google Scholar 

  23. Nohl H, Hegner D (1978) Do mitochondria produce oxygen radicals in vivo? Eur J Biochem 82:563–567

    PubMed  Google Scholar 

  24. Nohl H, Hegner D (1979) Responses of mitochondrial superoxide dismutase, catalase and glutathione peroxidase activites to aging. Mech Age Dev 11:145–151

    Google Scholar 

  25. Nohl H, Jordan W (1986) The mitochondrial site of superoxide formation. Biochem Biophys Res Commun 138:533–539

    PubMed  Google Scholar 

  26. Packer L, Deamer DW, Health RL (1967) Regulation and deterioration of structure in membranes. In: Strehler RL (ed) Advances in gerontological research. Academic Press, New York, pp 77–120

    Google Scholar 

  27. Perkins EH (1971) Phagocytic activity of aged mice. J Reticuloendothel Sec 9:642–643

    Google Scholar 

  28. Pryor WA (1984) Free radicals in autoxidation and aging. In: Armstrong D, Sohol RS, Cutler RG, Slater TF (eds) Free radicals in molecular biology, aging and disease. Raven Press, New York, pp 13–41

    Google Scholar 

  29. Recknagel RO, Ghoshal AK (1986) Lipoperoxidation as a vector in carbon tetrachloride hepatotoxicity. Lab Invest 15:132–146

    Google Scholar 

  30. Reiss U, Tappel AL (1973) Fluorescent product formation and changes in structure of DNA reacted with peroxidizing arachidonic acid. Lipids 8:199–205

    PubMed  Google Scholar 

  31. Rabatic S, Sabioncello A, DeKaris D, Kardum I (1988) Age-related changes in function of peripheral blood phagocytes 45:223–229

    Google Scholar 

  32. Sawada M, Carlson JC (1987) Changes in superoxide radical and lipid peroxide formation in the brain, heart and liver during the lifetime of the rat. Mech Ageing Dev 41:125–137

    PubMed  Google Scholar 

  33. Schoutsen B, Willem de Jong J (1987) Age-dependent increase in xanthine oxidoreductase differs in various heart cell types. Circ Res 61:604–607

    PubMed  Google Scholar 

  34. Shlafer M, Myers CL, Adkins S (1987) Mitochondrial hydrogen peroxide generation and activities of glutathione peroxidase and superoxide dismutase following global ischemia. J Mol Cell Cardiol 19:1195–1206

    PubMed  Google Scholar 

  35. Suzuki Y (1975) Studies on the free radicals in myocardial mitochondria by electron spin resonance (ESR) spectrometry (studies on experimental infarction dog). Jap Circ J 39:683–691

    PubMed  Google Scholar 

  36. Tappel AL (1975) Lipid peroxidation and fluorescent molecular damage to membranes. In: Trump BF, Arstila AV (eds) Pathology of cell membranes. Academic Press, New York, pp 145–170

    Google Scholar 

  37. Tappel AL, Fletcher B, Deamer D (1973) Effect of antioxidants and nutrients on lipid peroxidation fluorescent products and aging parameters in the mouse. J Gerontol 28:415–424

    PubMed  Google Scholar 

  38. Tietze F (1969) Enzymatic method for quantitative determination of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem 27:502–522

    PubMed  Google Scholar 

  39. Tsuchida M, Toshiyuki M, Mizutani K, Aibara K (1985) Fluorescent substances in mouse and human sera as a parameter of in vivo lipid peroxidation. Biochim Biophys Acta 834:196–204

    PubMed  Google Scholar 

  40. Williams AJ, Barrie SE (1985) Temperature effects on kinetic of calcium transport by cardiac mitochondria. Biochem Biophys Res Commun 1:123–128

    Google Scholar 

Download references

Author information

Author notes

  1. C. Muscari

    Present address: Centre of Research on Heart Metabolism, Department of Biochemistry, University of Bologna, via Irnerio 48, 40126, Bologna, Italy

Authors and Affiliations

  1. Centre of Research on Heart Metabolism, Department of Biochemistry, University of Bologna, via Irnerio 48, 40126, Bologna, Italy

    C. M. Caldarera & C. Guarnieri

Authors
  1. C. Muscari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. M. Caldarera
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Guarnieri
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Muscari, C., Caldarera, C.M. & Guarnieri, C. Age-dependent production of mitochondrial hydrogen peroxide, lipid peroxides and fluorescent pigments in the rat heart. Basic Res Cardiol 85, 172–178 (1990). https://doi.org/10.1007/BF01906970

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01906970

Key words

Search

Navigation