Basic Research in Cardiology

, Volume 73, Issue 2, pp 241–255 | Cite as

Electrically induced coronary artery thrombosis in closed chest anaesthetized dogs. Evaluation of the method

  • L. H. Snoeckx
  • K. Bruyneel
  • F. de Clerck
  • A. Verheyen
  • R. S. Reneman
Original Contributions

Summary

TheSalazar technique for electrical induction of coronary thrombosis, was used in 12 dogs, whereas a control procedure was followed in 6 other dogs.

Electrocardiographic, hemodynamic, biochemical and hematological studies were carried out before, during and 24 hours after electrical stimulation. Chaotic rhythm appeared in all electrically stimulated dogs 24 hours after thrombus formation.

Muscle necrosis was evident as indicated by the increase in SGOT and SGPT, but the observed hemodynamic changes were rather small. Light and electron microscopic studies of the coronary arteries showed important lesions in the vessel wall of the catheterized coronary arteries and an intracoronary thrombus was found in all the animals electrically stimulated as well as in two control dogs. These studies also revealed a multiple origin of coronary artery thrombus formation.

This closed chest model for acute myocardial infarction is well suitable for the study of early dysrhythmias, especially because of the high incidence of dysrhythmia and the low mortality rate. The model is not suitable to study new chemical compounds with anti-thrombotic activity, because of the multiple uncontrollable origin of the coronary thrombus.

Keywords

Acute Myocardial Infarction Thrombus Formation Coronary Thrombosis Closed Chest Coronary Thrombus 

Elektrisch induzierte Koronararterienthrombose bei Hunden mit geschlossenem Thorax. Bewertung der Methode

Zusammenfassung

Bei 12 Versuchshunden wurde die Technik nachSalazar zur Erzeugung von elektrisch induzierten Koronarthrombosen angewandt. Als Kontrolle dienten weitere 6 Hunde.

Elektrokardiographische, hämodynamische, biochemische und hämatologische Untersuchungen wurden vor, während und 24 Stunden nach elektrischer Reizung durchgeführt. Bei allen Versuchshunden (mit elektrischer Reizung) traten innerhalb von 2 Stunden nach Thrombusbildung Rhythmusstörungen auf.

Aufgrund des Anstiegs von SGOT und SGPT wurden Myokardnekrosen nachgewiesen, dagegen waren die beobachteten hämodynamischen Veränderungen sehr gering. Licht- und elektronenmikroskopische Untersuchungen der Koronararterien zeigten schwere Läsionen der Gefäßwände nach Katheterisierung der Koronararterien. Ein intrakoronarer Thrombus wurde bei allen Tieren nach Anwendung elektrischer Ströme gefunden, ebenso jedoch auch bei 2 Kontrollhunden. Diese Untersuchungen zeigen, daß für die Bildung von Thrombosen der Koronararterien unterschiedliche Ursachen verantwortlich zu machen sind.

Dieses Myokardinfarkt-Modell am nicht eröffneten Thorax ist zur Untersuchung frühzeitiger Rhythmusstörungen geeignet, besonders wegen des häufigen Auftretens der Dysrhythmien bei niedriger Mortalität. Das Modell erscheint jedoch nicht dafür geeignet, neue Arzneimittel mit antithrombotischer Wirkung zu testen, weil die Ursache der Thrombusbildung vielfältig und nicht kontrollierbar ist.

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Copyright information

© Dr. Dietrich Steinkopff Verlag 1978

Authors and Affiliations

  • L. H. Snoeckx
    • 1
    • 2
  • K. Bruyneel
    • 1
    • 2
  • F. de Clerck
    • 1
    • 2
  • A. Verheyen
    • 1
    • 2
  • R. S. Reneman
    • 1
    • 2
  1. 1.Life Sciences Department, Janssen Research LaboratoriesJanssen PharmaceuticaBeerse(Belgium)
  2. 2.Department of Physiology, Biomedical CenterUniversity of LimburgMaastricht(The Netherlands)

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