Summary
Using a combination of conventional mRNA-extraction methods and newer hybridization techniques, a method was developed which allows the quantitation of cardiac Poly(A)-containing mRNA in cardiac biopsies. This provides a new tool to directly assess changes in cardiac gene expression in cardiac biopsies. It is proposed that the method described may be valuable in investigations dealing with various human or animal heart diseases where changes in cardiac gene expression are expected and where only biopsy material is available.
Zusammenfassung
Durch Kombination konventioneller mRNS-Isolierungsverfahren mit neueren mRNS-Hybridisierungstechniken wurde eine Methode entwickelt, die die Quantifizierung myokardialer, Poly(A)-enthaltender mRNS in Myokardbiopsien ermöglicht. Dadurch wird es möglich, Veränderungen der myokardialen Genexpression direkt in Myokardbiopsien zu untersuchen. Die beschriebene Methode kann daher besonders nutzvoll sein, wenn Veränderungen der myokardialen Genexpression bei menschlichen oder tierischen Herzmuskelerkrankungen erwartet werden und wenn andererseits aber nur Herzmuskelbiopsien für die Untersuchungen zur Verfügung stehen.
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Zähringer, J., Stäb, G. & Pritzl, N. Quantitation of poly(A)-containing mRNA in rat cardiac biopsies. Basic Res Cardiol 78, 203–209 (1983). https://doi.org/10.1007/BF01906673
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DOI: https://doi.org/10.1007/BF01906673