Summary
A canine model for a standardized induction of collaterals is presented with a fixed external constrictor that is not designed to induce an occlusion of the coronary artery and at least over the timespan of 6 weeks does not impair perfusion under resting conditions in the myocardium-at-risk. The coronary constriction was standardized by a reduction of the postocclusive reactive hyperemia of 50%. Flow measurements were performed by flowmeter and by radioactive microspheres acutely and after an interval of 6 weeks of constriction. The results showed an increase of the collateral flow from 21.2±11.8 ml/100g/min−1 to 42.8±16.2 ml/100g/min−1 (p<0.05). The regional perfusion exhibited a transmyocardial gradient in favour of the subepicardial layers with 49.3±25 ml/100g/min−1 as compared to 33.1±17.3 ml/100g/min−1 (p<0.05) of the endocardial layers. Reactive hyperemia, as determined by flowmeter, was decreased by 21% after 6 weeks on account of slow progression of the coronary constriction due to intimal reactions, whereas reactive hyperemia, as determined by the microsphere method, increased by 9% due to additional collateral channels.
Zusammenfassung
Ein Hundemodell zur standardisierten Induktion von Kollateralen mit Hilfe eines externen Konstriktors wird vorgestellt, bei welchem eine Okklusion der Koronararterie nicht vorgesehen ist und wenigstens über 6 Wochen die Myokardperfusion in Ruhe nicht eingeschränkt wird. Flußmessungen wurden mit einem elektromagnetischen Flowmeter und mit radioaktiven Mikrosphären im akuten Versuch durchgeführt und nach 6 Wochen wiederholt. Die Ergebnisse zeigten eine Zunahme des Kollateralflusses von 21,2±11,8 ml/100g/min−1 auf 42,8±16,2 ml/100 g/min−1 (p<0.05). Die regionale Durchblutung wies einen transmyokardialen Gradienten auf, welcher die subepikardialen Schichten gegenüber den subendokardialen bevorzugt: 49,3±25 ml/100 g/min−1 gegenüber 33,1±17,3 ml/100 g/min−1 (p<0,05). Die mit dem Flowmeter gemessene reaktive Hyperämie nahm über 6 Wochen um 21% ab, bedingt durch eine Verengung der Stenosen aufgrund von Intimareaktionen im Konstriktorbereich, während die mit Mikrosphären gemessene Durchblutung aufgrund zusätzlicher Kollateralversorgung um 9% zunahm.
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References
Blumgart, H. L., P. M. Zoll, A. S. Freedberg, D. R. Gilligan: The experimental production of intercoronary arterial anastomoses and their functional significance. Circulation1, 10 (1950).
Lumb, G. D., L. B. Hardy: Collateral circulation and survival related to gradual occlusion of the right coronary artery in the pig. Circulation27, 717 (1963).
Meesmann, W.: Untersuchungen zur Funktion der intra-arteriellen Koronaranastomosen beim Herzinfarkt. Z. Kreislaufforsch.48, 193 (1959).
Schaper, W.: The Collateral Circulation of the Heart. North Holland Publishing Co., Amsterdam, London, American Elsevier Publishing Co. Inc. (New York 1971).
Hecht, H. S., J. M. Aroesty, P. I. Laraia, E. Markin, S. Paulin: Role of coronary collateral circulation in the preservation of left ventricular function. Circulation48 (Suppl. IV), 344 (1973).
Helfant, R. H., P. S. Vokonas, R. Gorlin: Functional importance of human coronary collateral circulation. New Engl. J. Med.284, 1277 (1971).
Helfant, R. H., H. G. Kemp, R. Gorlin: Coronary atherosclerosis, coronary collaterals and their relation to cardiac function. Ann. Intern. Med.73, 189 (1970).
Schaper, W.: Experimental coronary artery occlusion. III. The determinants of collateral blood flow in acute coronary occlusion. Basic Res. Cardiol.73, 584–594 (1978).
Levin, D. C., M. Kauff, H. A. Baltaxe: Coronary collateral circulation. Amer. J. Röntgenol. Rad. Ther. and Nucl. Med.119, 463–473 (1973).
Scheel, K. W.: The stimulus for coronary growth. Ischemia or pressure difference? Circulation60, Suppl. II, 393 (1979).
Hirzel, H. O., G. R. Nelson, E. H. Sonnenblick, E. S. Kirk: Redistribution of collateral blood flow from necrotic to surviving myocardium following coronary occlusion. Circulat. Res.39, 214–222 (1976).
Kirk, E. S., H. O. Hirzel, E. H. Sonnenblick: Primary importance of myocardial blood flow in determining the survival of ischemic myocardium, pp. 196–201, in: Coronary Heart Surgery: A Rehabilitation Measure, Roskamm, H., M. Schmuziger (Eds.), Springer (Berlin, Heidelberg, New York 1979).
Kalbfleisch, H.: Eine Methode zur postmortalen Größenbestimmung der Versorgungsgebiete einzelner Herzkranzarterien. Z. Kardiol.64, 987 (1969).
Schaper, W., W. Flameng, B. Winkler, B. Wüsten, W. Türschmann, G. Neugebauer, M. Carl, S. Pasyk: Quantification of collateral resistance in acute and chronic experimental coronary occlusion in the dog. Circulat. Res.39, 371 (1976).
Snedecor, G. W., G. W. Cochran: Statistical Methods, 2nd Edition. Iowa State University Press (Ames, Iowa, 1967).
Wüsten, B.: The degree of coronary stenosis as determinant of collateral development. In: The Pathophysiology of Myocardial Perfusion, pp. 453–470, W. Schaper (Ed.), Elsevier/North-Holland Biomedical Press (Amsterdam, New York, Oxford 1979).
Millard, R. W.: Coronary collateral development to viable porcine myocardium. XXVIIIth International Congress on Physiological Scienses. Satellite Symposium on Coronary Circulation, Bad Nauheim, West Germany 1980.
Schaper, W.: The measurement of regional myocardial perfusion. In: The Pathophysiology of Myocardial Perfusion, pp. 9–11, W. Schaper (Ed.), Elsevier/North-Holland Biomedical Press (Amsterdam, New York, Oxford 1979).
Gottwik, M. G., E. S. Kirk, S. Hoffstein, W. B. Weglicki: Effect of collateral flow on epicardial and endocardial lysosomal hydrolases in acute myocardial ischemia. J.C.I.56, 914–923 (1975).
Fulton, W. F. M.: The Coronary Arteries. C. G. Thomas (Springfield, Ill. 1956).
Wüsten, B., J. Schaper, M. Gottwik, B. Farohs, W. Schaper: Chronic experimental coronary stenosis: hemodynamic and morphological findings. Poster and Abstract: XXVIII: 21st Internat. Congress of Physiological Sciences, Satellite Symposium on Coronary Circulation, Bad Nauheim, West Germany 1980.
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Gottwik, M., Wüsten, B., Hofmann, M. et al. Development of collaterals. Application of external subcritical fixed constrictors in a canine model. Basic Res Cardiol 78, 183–192 (1983). https://doi.org/10.1007/BF01906671
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DOI: https://doi.org/10.1007/BF01906671