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Basic Research in Cardiology

, Volume 70, Issue 6, pp 661–670 | Cite as

Purkinje fibre glycogen a morphologic and biochemical study of glycogen particles isolated from the cow conducting system

  • L. -E. Thornell
  • M. Sjöström
Original Contributions

Summary

Glycogen from the cow conducting system was extracted by crude and mild methods. For comparison similar extractions were also performed on cow ordinary ventricular tissue. Glycogen particles from the conducting system, isolated by a mild method, were characterized by a low molecular weight (3–5×106) and small dimensions (average diameter about 30 nm). 3.5–7% protein was firmly bound to the glycogen. The glycogen, based on spectrophotometric analysis, appeared to be in a native state. Glycogen as a polysaccharide-protein complex can thus be obtained from the cow conducting system and is proposed to be useful for analysis of the structure and function of glycogen in the conducting system.

Keywords

Public Health Molecular Weight Native State Average Diameter Biochemical Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Glykogen aus Purkinje-Fasern Morphologische und biochemische Untersuchungen von Glykogenpartikeln aus dem Reizleitungssystem der Kuh

Zusammenfassung

Aus dem Reizleitungssystem von Kuhherzen wurde Glykogen sowohl mit schonenden als auch mit groben Methoden extrahiert. Vergleichbare Extraktionen wurden ebenso aus normalem Ventrikelgewebe durchgeführt. Die auf schonende Weise isolierten Glykogenpartikel des Reizleitungssystems waren gekennzeichnet durch ein geringes Molekulargewicht (3–5·106) und durch geringe Ausmaße (durchschnittlicher Durchmesser ungefähr 30 nm). 3,5–7% Protein war fest am Glykogen gebunden. Die spektrophotometrische Analyse wies auf einen natürlichen Zustand des Glykogens hin. Daher erscheint die hier beschriebene Isolierung des Polysaccharid-Komplexes aus dem Reizleitungssystem der Kuh für weitere Untersuchungen von Struktur und Funktion des Glycogens im Reizleitungssystem geeignet.

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Copyright information

© Dr. Dietrich Steinkopff Verlag 1975

Authors and Affiliations

  • L. -E. Thornell
    • 1
  • M. Sjöström
    • 1
  1. 1.Department of AnatomyUniversity of UmeåUmeå(Sweden)

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