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The release of calcium from cardiac mitochondria: The importance of the calcium-protein ratio

Kalzium-Freisetzung aus Herzmitochondrien: Das Kalzium-Mitochondrienprotein-Verhältnis als entscheidendes Kriterium

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Summary

Using a DW 2 dual wavelength spectrophotometer (AMINCO) and murexide as a Ca2+ sensitive indicator it is shown that guinea-pig heart mitochondria can release accumulated Ca2+ without the influence of non-physiological material. The main factor which decides if accumulated Ca2+ is spontaneously released is the Ca2+/mitochondrial-protein ratio. Under appropriate assay conditions a critical intramitochondrial Ca2+ concentration is reached. Increasing this concentration leads to Ca2+-release.

Zusammenfassung

Mit Hilfe eines Doppelwellenlängen-Photometers (DW-2, AMINCO) und von Murexid als Ca2+-Indikator konnte gezeigt werden, daß Meerschweinchen-Herz-mitochondrien auch ohne den Zusatz unphysiologischer Agenzien (wie Entkopplern oder Ruthenium Rot) gespeichertes Calcium spontan wieder freisetzen können. Entscheidend für diese spontane Ca2+-Freisetzung ist das Verhältnis von Calcium und Mitochondrienprotein. Unter bestimmten definierten Bedingungen stellt sich eine kritische intramitochondriale Ca2+-Konzentration ein; wird dieser Schwellenwert überschritten, tritt spontane Ca2+-Freisetzung ein.

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Author notes

  1. G. G. Göring & P. G. Spieckermann

    Present address: Physiologisches Institut, Lehrstuhl I der Universität Göttingen (BRD), Humboldtallee 7, D-3400, Göttingen, W. Germany

  2. W. G. Nayler

    Present address: Cardiothoracic Institute, University of London, 2. Beaumont Street, W1N 2 DX, London, England

Authors and Affiliations

  1. Physiologisches Institut, Lehrstuhl I der Universität Göttingen (BRD), Humboldtallee 7, D-3400, Göttingen, W. Germany

    W. G. Nayler

Authors
  1. G. G. Göring
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  2. W. G. Nayler
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  3. P. G. Spieckermann
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Additional information

With 2 figures and 1 table

Supported by the Deutsche Forschungsgemeinschaft-SFB 89-Kardiologie, Göttingen, the British Heart Foundation and the Medical Research Council of Great Britain.

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Göring, G.G., Nayler, W.G. & Spieckermann, P.G. The release of calcium from cardiac mitochondria: The importance of the calcium-protein ratio. Basic Res Cardiol 72, 77–81 (1977). https://doi.org/10.1007/BF01906303

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  • DOI: https://doi.org/10.1007/BF01906303

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