Summary
A vesicular microsomal fraction isolated from hog fundic mucosa demonstrates the capacity to take up equal amounts of RB+ and Cl−. The amount of the Rb+ uptake is sensitive to the extravesicular osmolarity, and rate of uptake is sensitive to temperature.86Rb+ efflux is dependent upon the cation composition of the diluting solution. ATP, but not β-γ methylene ATP, induces a reversible efflux of86Rb+ from loaded vesicles, and this is dependent upon a functional K+-ATPase. The ATP induced efflux is not affected by CCCP (carbonyl cyanidem-chlorophenylhydrazone) or TCS (tetrachlorosalicylanilide) nor by lipid soluble ions or valinomycin. Nigericin inhibits the efflux by 40%. Uptake of the lipid soluble ion14C-SCN− has been demonstrated and is enhanced by ATP only in the presence of valinomycin. The results are consistent with a neutral or isopotential exchange of H+ for Rb+ mediated by K+-ATPase.
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Schackmann, R., Schwartz, A., Saccomani, G. et al. Cation transport by gastric H+∶K+ ATPase. J. Membrain Biol. 32, 361–381 (1977). https://doi.org/10.1007/BF01905228
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DOI: https://doi.org/10.1007/BF01905228