Summary
The interaction of 1-anilino-8-naphthalene-sulfonate (ANS) with vesicles derived from hog fundic mucosa was studied in the presence of valinomycin and with the addition of ATP. Evidence was found for two classes of sites, those rapidly accessible to ANS with aK D of 7.5 μm and those slowly accessible, but rapidly accessed in the presence of valinomycin with aK D of 2.5 μm. ATP transiently increases the quantum yield of the latter ANS binding sites only in the presence of valinomycin, but does not alter the number ofK D of those sites. The time course of this increase correlates with H+ uptake and Rb+ extrusion by those vesicles and H+ carriers such as tetrachlorsalicylanilide or nigericin abolish the ATP response. With ATP addition in the presence of SC14N and valinomycin there is transient uptake of SCN−. It is concluded that ANS is acting as a probe of a structural change dependent on a potential and H+ gradient.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Azzi, A., Chance, B., Radda, G.K., Lee, C.P. 1969a. A fluorescence probe of energy dependent structure changes in fragmented membranes.Proc. Nat. Acad. Sci. USA 62:612
Azzi, A., Gherardini, P., Santato, P. 1971. Fluorochrome interaction with the mitochondrial membrane; the effect of energy conservation.J. Biol. Chem. 246:2035
Azzi, A., Fleischer, S., Chance, B. 1969b. Cytochromec phospholipid interaction: Structural transitions associated with valency changes.Biochem. Biophys. Res. Commun. 36:322
Bakker, E.P., Van Dam, K. 1974. The influence of diffusion potentials across liposomal membranes on the fluorescence intensity of 1-anilino-naphthalene-8-sulphonate.Biochim. Biophys. Acta 339:157
Brocklehurst, J.R., Freedman, R.B., Hancock, D.J., Radda, G.K. 1970. Membrane studies with polarity-dependent and excimer-forming fluorescent probes.Biochem. J. 116:721
Chance, B. 1970. Fluorescent probe environment and the structural and charge changes in energy coupling of mitochondrial membranes.Proc. Nat. Acad. Sci. USA 67:560
Chance, B., Lee, C. 1969. Comparison of fluorescence probe and light-scattering readout of structural states of mitochondrial membrane fragments.FEBS Lett. 4:181
Feinstein, M.B., Felsenfeld, H. 1971. The detection of ionophorous antibiotic-cation complexes in water with fluorescent probes.Proc. Nat. Acad. Sci. USA 68:2037
Forte, J.G., Ganser, A., Beesley, R., Forte, T.M. 1975. Unique enzymes of purified microsomes from pig fundic mucosa; K+-stimulated adenosine triphosphate and K+-stimulated pNPPase.Gastroenterology 69:175
Forte, J.G., Ganser, A.L., Tanisawa, A.S. 1974. The K+-stimulated ATPase system of microsomal membranes from gastric oxyntic cells.Ann. N.Y. Acad. Sci. 242:255
Fortes, P.A.G. 1976. Nanosecond fluorescence spectroscopy of biological membranes.In: Mitochondria: Bioenergetics, Biogenesis and Membrane Structure. Packer and Gomez-Puyou, editors. Academic Press, N.Y., pp. 327–348
Friedman, R.B., Radda, G.K. 1969. The interaction of 1-anilino-8-naphthalene sulfonate with erythrocyte membranes.FEBS Lett. 3:150
Ganser, A.L., Forte, J.G. 1973a. K+ stimulated ATPase in purified microsomes of bullfrog oxyntic cells.Biochim. Biophys. Acta 307:169
Ganser, A.L., Forte, J.G. 1973b. Ionophoretic stimulation of K+-ATPase of oxyntic cell microsomes.Biochem. Biophys. Res. Commun. 54:690
Jasaitis, A.A., Kuliene, V.V., Skulachev, V.P. 1971. Anilinonaphthalenesulfonate fluorescence changes induced by non-enzymatic generation of membrane potential in mitochondria and submitochondrial particles.Biochim. Biophys. Acta 234:177
Lee, J., Simpson, G., Scholes, P. 1974. An ATPase from dog gastric mucosa: Changes of outer pH in suspensions of membrane vesicles accompanying ATP hydrolysis.Biochem. Biophys. Res. Commun. 60:825
Markin, V.S., Sokolov, V.S., Boguslavsky, L.I., Jaguzhinsky, L.S. 1975. Nigericin-induced charge transfer across membranes.J. Membrane Biol. 25:23
Masotti, L.F., Long, M.M., Sachs, G., Urry, D.W. 1972. The effect of ATP on the CD spectrum of membrane fraction from oxyntic cells.Biochim. Biophys. Acta 255:420
Rubalcava, B., deMunoz, D.M., Gitler, C. 1969. Interaction of fluorescent probes with membranes. I. Effect of ions on erythrocyte membranes.Biochemistry 8:2742
Saccomani, G., Stewart, H.B., Shaw, D., Lewin, M., Sachs, G. 1977. Fractionation and purification of K+ ATPase containing vesicles by zonal centrifugation and free flow electrophoresis technique.Biochim. Biophys. Acta (In press)
Saccomani, G., Shah, G., Spenney, J.G., Sachs, G. 1975. Characterization of gastric mucosal membranes. VIII. The localisation of peptides by iodination and phosphorylation.J. Biol. Chem. 250:4802
Sachs, G., Rabon, E., Chang, H., Schackmann, R., Lewin, M., Saccomani, G. 1976. A nonelectrogenic H+ pump in plasma membranes of hog stomach.J. Biol. Chem. 251:7690
Sachs, G., Saccomani, G., Rabon, E., Sarau, H.M. 1975. Redox and ATP in acid secretion.Ann. N.Y. Acad. Sci. 264:456
Spenney, J.G., Saccomani, G., Spitzer, H.L., Tomana, M., Sachs, G. 1974a. Characterization of gastric mucosal membranes; composition of gastric cell membranes and polypeptide fractionation using ionic and nonionic detergents.Arch. Biochem. Biophys. 161:456
Spenney, J.G., Strych, A., Price, A.H., Helander, H.F., Sachs, G. 1974b. Preparation of gastric cell membranes by zonal density gradient centrifugation.Method. Dev. Biochem. 4:309
Stryer, L. 1965. The interaction of a naphthalene dye with apomyoglobin and apohemoglobin; a fluorescent probe of non-polar binding sites.J. Molec. Biol. 13:482
Tanisawa, A.S., Forte, J.G. 1971. Phosphorylated-intermediate of microsomal ATPase from rabbit gastric mucosa.Arch. Biochem. Biophys. 147:165
Vanderkooi, J.M., Martonosi, A. 1971. Sarcoplasmic reticulum. XII. The interaction of 8-anilino-1-naphthalene sulfonate with skeletal muscle microsomes.Arch. Biochem. Biophys. 144:87
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lewin, M., Saccomani, G., Schackmann, R. et al. Use of 1-anilino-8-naphthalene-sulfonate as a probe of gastric vesicle transport. J. Membrain Biol. 32, 301–318 (1977). https://doi.org/10.1007/BF01905224
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01905224