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On the ultrastructure of follicles and isolated follicular granulosa cells of porcine ovary

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Summary

The endoplasmic reticulum in granulosa cells of primary, secondary, and small tertiary follicles of the porcine ovary is sparse and largely of the granular type.

In granulosa cells of large tertiary follicles the endoplasmic reticulum shows distinct signs of proliferation. Some cells even contain whorls of endoplasmic reticulum membranes, essentially of the agranular variety.

Direct continuity between endoplasmic reticulum membranes of the granular and agranular type as well as the continuous increase in agranular membranes suggest that these membranes may originate from the granular membranes.

Granulosa cells isolated from large tertiary follicles by microdissection and keptin vitro show essentially the same ultrastructure as granulosa cells of intact large tertiary follicles.

Some lipid droplets appear to be localized in cavities of the endoplasmic reticulum. It is suggested that the droplets contain precursor material for steroid hormone synthesis.

Finally, the development of the agranular endoplasmic reticulum including the appearance of whorls in some granulosa cells of large tertiary follicles indicates that steroid synthesis may occur in such follicular granulosa cells.

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Authors and Affiliations

  1. Institute of Pathology, University of Umeå, Umeå 6, Sweden

    Lars Bjersing

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  1. Lars Bjersing
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Additional information

Read at the Meeting of the Swedish Society for Pathology in Umeå, September 25, 1965 (Bjersing, 1966).

This investigation was supported by grants from the Swedish Medical Research Council (Projects No. 13 X-78-01, 12 X-78-02, and 12 X-78-03).

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Bjersing, L. On the ultrastructure of follicles and isolated follicular granulosa cells of porcine ovary. Z.Zellforsch 82, 173–186 (1967). https://doi.org/10.1007/BF01901700

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  • DOI: https://doi.org/10.1007/BF01901700

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