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The effect of oxygen concentration on the quantum yields of the dye-sensitized photoinactivation of trypsin, α-chymotrypsin and lysozyme

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  • Chimica, Biochimica, Biophysica
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Résumé

Le travail décrit l'effet de différentes concentrations en oxygène sur la photoinactivation de la trypsine, de l'α-chymotrypsine et du lysozyme. Comme sensibilisateurs on a utilisé le bleu de méthylène, l'éosine Y et le FMN. En général, les rendements quantiques de l'inactivation enzymatique ont augmenté avec l'augmentation des concentrations en oxygène, et atteint un plateau à des concentrations de 20% environ. Ils ne changèrent alors pratiquement plus lorsque les concentrations en oxygène augmentèrent jusqu'à 100%.

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Authors and Affiliations

  1. Department of Biology, University of Utah, 84112, Salt Lake City, Utah, USA

    C. F. Hodgson, E. B. McVey & J. D. Spikes

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  1. C. F. Hodgson
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  2. E. B. McVey
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  3. J. D. Spikes
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Additional information

This work was supported by the U.S. Atomic Energy Commission under Contract No. AT(11-l)-875.

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Hodgson, C.F., McVey, E.B. & Spikes, J.D. The effect of oxygen concentration on the quantum yields of the dye-sensitized photoinactivation of trypsin, α-chymotrypsin and lysozyme. Experientia 25, 1021–1022 (1969). https://doi.org/10.1007/BF01901399

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  • DOI: https://doi.org/10.1007/BF01901399

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