Summary
A technique for cryostat sectioning of fresh plant tissues was developed. Embedding in unfixed brain pieces prevents disruption of plant tissue during freezing and sectioning. The method is now applied to histochemical studies on the distribution of minerals in plant tissues.
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Läuchli, A. Kryostatschnittechnik für natives Pflanzengewebe. Experientia 22, 335–336 (1966). https://doi.org/10.1007/BF01900487
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DOI: https://doi.org/10.1007/BF01900487