Advertisement

Experientia

, Volume 26, Issue 3, pp 331–335 | Cite as

Verbesserte Methode zur Gewinnung und Verarbeitung von freien Zellen aus kleinsten Volumina von Körperflüssigkeiten mittels Membranfiltration

  • G. Kistler
  • W. Scherle
  • A. Hauswirth
Specialia Pro Experimentis

Summary

A cell collector for concentrating small numbers of exfoliated cells on membrane filters from minute volumes (0.05–3 ml) of body fluids and culture media is described. The apparatus incorporates a membrane pump, delivering a vacuum of 10–100 mm Hg. Filtration units (funnels and filter holders) with filtrating areas of 3, 5 and 8 mm diameter are used depending on the volume and the cell content of the fluid to be investigated. A rapid staining procedure based on the classical May-Grünwald-Giemsa technique for light microscopic observations and a modified technique for processing the cell-coated filters for electron microscopy are presented in some detail.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Birkhäuser-Verlag 1970

Authors and Affiliations

  • G. Kistler
    • 1
  • W. Scherle
    • 1
  • A. Hauswirth
    • 1
  1. 1.Elektronenmikroskopische Abteilung des Anatomischen Institutes der UniversitätZürichSchweiz

Personalised recommendations