Verbesserte Methode zur Gewinnung und Verarbeitung von freien Zellen aus kleinsten Volumina von Körperflüssigkeiten mittels Membranfiltration
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A cell collector for concentrating small numbers of exfoliated cells on membrane filters from minute volumes (0.05–3 ml) of body fluids and culture media is described. The apparatus incorporates a membrane pump, delivering a vacuum of 10–100 mm Hg. Filtration units (funnels and filter holders) with filtrating areas of 3, 5 and 8 mm diameter are used depending on the volume and the cell content of the fluid to be investigated. A rapid staining procedure based on the classical May-Grünwald-Giemsa technique for light microscopic observations and a modified technique for processing the cell-coated filters for electron microscopy are presented in some detail.
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