Summary
Gibberellic acid (GA) when applied alone is without effect in theAvena curvature test. When applied together with indole-3-acetic acid (IES=IAA; 10−8–10−5 g/ml, GA increases the extent of the IAA-induced curvature by about 40%; this is true with all IAA concentrations including supraoptimal ones. It is computed that the stimulation by GA results from a growth stimulation in both halves of the coleoptile; in the convex as well as the concave side. The GA stimulation cannot be explained by an effect on the metabolism of IAA.
DecapitatedAvena-coleoptiles received a phototropic stimulus (2. positive curvature range); after repeated decapitations IAA and/or GA were applied to the whole cut surface. Without IAA, the tropistic curvature is small, and GA is without effect. IAA increases the tropistic response. When applied together with IAA, GA increases the curvature by about 40%.
In both tests (Avena curvature and phototropism) the response is quantitatively the same with all GA concentrations (10−9–10−4 g/ml).
From experiments in which GA was applied only to one side of the cut surface, IAA to the other or to both sides (both sides separated by tinfoil), it is concluded that GA is rapidly transported in lateral direction through theAvena coleoptile. The rapid transverse transport through stem sections was demonstrated by applying GA to one side ofPisum epicotyls or ofHelianthus hypocotyls, and collecting the transported GA in agar blocks at the other side, and testing it with dwarf pea plants. This lateral transport cannot be a physical diffusion, because it is blocked by triiodobenzoic acid.
Gibberellins cannot be a link in the reaction chain from tropistic stimulus to reaction; they do not influence tropisms directly, but may be necessary for an optimal curvature by securing optimal growth.
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Libbert, E., Gerdes, I. Können Gibberelline an tropistischen Krümmungen beteiligt sein?. Planta 61, 245–258 (1964). https://doi.org/10.1007/BF01897159
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DOI: https://doi.org/10.1007/BF01897159