The plant growth retardant CCC as inhibitor of gibberellin biosynthesis inFusarium moniliforme
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Gibberellin (GA) production inFusarium moniliforme (Gibberella fujikuroi) is suppresed by adding the plant growth retardant CCC [(2-chloroethyl)trimethylammonium chloride] to the culture medium. A concentration of 0.1 mg/l of CCC causes 50% inhibition whereas 10 mg/l and higher concentrations fully suppress GA production. Dry weight of the mycelium is not, or only slightly reduced in the presence of CCC.
Thin-layer chromatography of acidic fractions of CCC-free cultures reveals fluorescent spots at 4 differentR f values. No fluorescent spots can be detected on chromatograms of acidic fractions obtained from CCC cultures, thus demonstrating that production of all GA's is inhibited by CCC.
If CCC is added to the medium 2 or 3 days after inoculation, further GA production is blocked, but the level of GA present at the time of CCC application is maintained. CCC does not enhance inactivation of GA3 in sterile culture medium, nor in the presence of the fungus. It is therefore concluded that CCC inhibits the biosynthesis of GA in the fungus.
Transfer of thoroughly washed mycelium from medium with CCC to fresh medium does not result in GA production because sufficient CCC is carried over in the mycelium to block GA biosynthesis completely.
KeywordsChloride Chromatography Plant Growth Fresh Medium Gibberellin
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