Synopsis
The existence of disulphide groups in the granules of the secretory portion of the ducts of rat submandibular glands has been demonstrated with methods that reveal thiol groups formed after reducing the disulphide groups first. Disulphide groups were also demonstrated with cationic dyes by staining the cysteic acid residues obtained after oxidation with permanganate. Alcian Blue at pH 3.0 was used for this purpose. Two kinds of granules, characterized by their reactions with Alcian Blue at different pH levels, were apparent in differing stages of the same secretion.
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Montero, C. Histochemistry of protein-bound disulphide groups in the duct secretory granules of the rat submandibular gland. Histochem J 4, 259–266 (1972). https://doi.org/10.1007/BF01890997
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DOI: https://doi.org/10.1007/BF01890997