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An examination of factors affecting the efficiency ofAgrobacterium-mediated transformation of tomato

Plant Cell Reports volume 16, pages 235–240 (1996)Cite this article

Abstract

An improved protocol forAgrobacterium-mediated transformation of the tomato cultivar Moneymaker was developed by examining the effects of six different factors on the efficiency of transformation. Explant size, explant orientation, gelling agent and plate sealant were found to affect transformation efficiency. Two other factors, type of explant (hypocotyl or cotyledon) and frequency of transfer to fresh selective regeneration medium, did not have any effect on transformation efficiency. By combining the best treatments for each factor, an average transformation efficiency of 10.6% was obtained for Moneymaker.

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References

  • Church GM, Gilbert W (1984) Proc Natl Acad Sci USA 81:1991

    PubMed  Google Scholar 

  • Chyi Y-S, Phillips GC (1987) Plant Cell Rep 6:105–108

    Google Scholar 

  • Davis ME, Lineberger RD, Miller AR (1991) Plant Cell Tiss Org Cult 24:115–121

    Article  Google Scholar 

  • Fillatti JJ, Kiser J, Rose R, Comai L (1987) Bio/Technology 5:726–730

    Article  Google Scholar 

  • Fulton TM, Chunwongse J, Tanksley, SD (1995) Pl Mol Biol Rep 13:207–209

    Google Scholar 

  • Hamza S, Chupeau Y (1993) J Exp Bot 44:1837–1845

    Google Scholar 

  • Hasegawa A, Verver J, Shimada A, Saito M, Goldbach R, van Kammen A, Miki K, Kameya-Iwaki M, Hibi T (1989) NAR 17:9993–10013

    PubMed  Google Scholar 

  • Jefferson RA, Kavanaugh TA, Bevan, MW (1987) EMBO J 6:3901–3907

    PubMed  Google Scholar 

  • McCormick S, Niedermeyer J, Fry J, Barnason A, Horsch R, Fraley R (1986) Plant Cell Rep 5:81–84

    Article  Google Scholar 

  • McCormick S (1991) In: Lindsey K (ed) Plant Tissue Culture Manual: Fundamentals and Applications. Kluwer Acad Publ, Dordrecht The Netherlands, pp B6/1–9

    Google Scholar 

  • Metz TD (1994) Ph.D. Dissertation, Cornell University, Ithaca,

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–497

    Google Scholar 

  • Nitsch JP (1969) Phytomorphology 19:389–404

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, Cold Spring Harbor, NY

    Google Scholar 

  • Shahin EA, Sukhapinda K, Simpson RB, Spivey R (1986) Theor Appl Genet 72:770–777

    Article  Google Scholar 

  • Thomas BR, Pratt D (1981) Theor Appl Genet 59:215–219

    Google Scholar 

  • Van Eck JM (1993) Ph.D. Dissertation, Cornell University, Ithaca, NY

  • van Roekel JS, Damm B, Melchers LS, Hoekema A (1993) Plant Cell Rep 12:644–647

    Article  Google Scholar 

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Affiliations

  1. Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853, Ithaca, NY, USA

    Anne Frary & Elizabeth D. Earle

Authors
  1. Anne Frary
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  2. Elizabeth D. Earle
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Communicated by J. M. Widholm

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Frary, A., Earle, E.D. An examination of factors affecting the efficiency ofAgrobacterium-mediated transformation of tomato. Plant Cell Reports 16, 235–240 (1996). https://doi.org/10.1007/BF01890875

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  • DOI: https://doi.org/10.1007/BF01890875

Keywords

  • Good Treatment
  • Transformation Efficiency
  • Regeneration Medium
  • Tomato Cultivar
  • Selective Regeneration