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Plant Cell Reports

, Volume 16, Issue 3–4, pp 167–173 | Cite as

High regeneration rates in anther culture of interspecific sunflower hybrids

  • Tengku Nurhidayah
  • Renate Horn
  • Thomas Röcher
  • Wolfgang Friedt
Article

Abstract

Optimization of anther culture with regard to the induction of callus formation and direct embryogenesis was obtained for interspecific hybrids ofH. annuus withH. tuberosus, H. laetiflorus, andH. resinosus by investigating six different induction media and four regeneration media. One media combination (MS-13, MS-R3 and MS-R4) used under different culture conditions (30°C / 35°C and different dark treatments) gave up to 92.7% embryogenic anthers with an average of 8.5 embryos per anther. However, direct embryogenesis as well as callus formation showed a strong genotypec and treatment specific reaction. From 5,600 anthers of the four investigated genotypes more than 2,000 plants could be regenerated. Regenerants were characterized by morphological traits and isozyme analyses to prove their androgenetic origin.

Keywords

Culture Condition Morphological Trait Induction Medium Regeneration Medium Callus Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BAP

6-benzylaminopurine

MNR

menadione reductase

NAA

α-naphthalene acetic acid

PGM

phosphoglucomutase

PGI

phospoglucoisomerase

PVP

polyvinylpyrrolidone

CMS

cytoplasmic male sterile

MS

Murashige & Skoog

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Tengku Nurhidayah
    • 1
  • Renate Horn
    • 1
  • Thomas Röcher
    • 1
  • Wolfgang Friedt
    • 1
  1. 1.Institut für Pflanzenbau and Pflanzenzüchtung IJustus-Liebig-UniversitätGiessenGermany

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