Plant Cell Reports

, Volume 16, Issue 3–4, pp 167–173 | Cite as

High regeneration rates in anther culture of interspecific sunflower hybrids

  • Tengku Nurhidayah
  • Renate Horn
  • Thomas Röcher
  • Wolfgang Friedt


Optimization of anther culture with regard to the induction of callus formation and direct embryogenesis was obtained for interspecific hybrids ofH. annuus withH. tuberosus, H. laetiflorus, andH. resinosus by investigating six different induction media and four regeneration media. One media combination (MS-13, MS-R3 and MS-R4) used under different culture conditions (30°C / 35°C and different dark treatments) gave up to 92.7% embryogenic anthers with an average of 8.5 embryos per anther. However, direct embryogenesis as well as callus formation showed a strong genotypec and treatment specific reaction. From 5,600 anthers of the four investigated genotypes more than 2,000 plants could be regenerated. Regenerants were characterized by morphological traits and isozyme analyses to prove their androgenetic origin.


Culture Condition Morphological Trait Induction Medium Regeneration Medium Callus Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.





menadione reductase


α-naphthalene acetic acid








cytoplasmic male sterile


Murashige & Skoog


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  1. Alissa A, Sericys H, Jonard R (1985) Physiologic végétale, C R Acad Sc Paris, 300. Série III, 1, 25–30Google Scholar
  2. Atalagic J, Dozet B, Skoric D (1993) Plant Breeding 111: 318–324Google Scholar
  3. Barth S, Voeste D, Schnabl H (1993) Bot Acta 106:100–102Google Scholar
  4. Bohorova N, Atanassov A, and Georgieva-Todorova J (1985) Z Pflanzenzüchtung 95: 35–44Google Scholar
  5. Colby LW and Peirce LC (1988) Hortscience 23:761–763Google Scholar
  6. Coumans M, Zhong D (1995) Plant Cell, Tissue and Organ Culture 41: 203–209Google Scholar
  7. Dahlhoff M, Köhler H, Friedt W (1992) In: Proc. of the 13th International Sunflower Conf Pisa, Italy, Vol II, pp 1438–1443Google Scholar
  8. Friedt W, Nurhidayah T, Röcher T, Köhler H, Bergmann R, Horn R (1996) In: Jain SM, Sopory SK, Veilleux RE (eds)In vitro haploid production in higher plants. Kluwer Acad Publ (in press)Google Scholar
  9. Gürel A, Nichterlein K, Friedt (1991) Plant Breeding 106:68–76Google Scholar
  10. Jonard R, Mezzarobba A (1990) In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry 10, Springer Verlag, Berlin, Heidelberg, pp 485–501Google Scholar
  11. Krasnyanski S, Menczel L (1994) Plant Cell Reports 14:232–235Google Scholar
  12. Kräuter R, Steinmetz A, Friedt W (1991) Theor Appl Genet 82:521–525CrossRefGoogle Scholar
  13. Mezzarobba A (1988) L'androgenèse chez le Tournesol, Thèse USTL, MontpellierrGoogle Scholar
  14. Mezzarobba A, Jonard R (1986) Physiologic végétale CR Acad Sci Paris, 303 III:181–186Google Scholar
  15. Mix G (1985) Antheren- and Ovarienkultur von Sonnenblumen (Helianthus annuus L.). Landbauforschung Völkenrode 3: 153–156Google Scholar
  16. Murashige T, Skoog I (1962) Physiol Plant 15:473–497Google Scholar
  17. Nenova N, Ivanov P, Christov M (1992) In: Proc 13th Int Sunfl Conf Pisa, Itlay, Vol II, pp 1509–1514Google Scholar
  18. Rieseberg LH, Soltis DE (1989) Aliso 12:277–286Google Scholar
  19. Schmidt T, Boblenz K, Metzlaff M, Kaemmer D, Weising K, Kahl G (1993) Theor Appl Genet 85: 635–657CrossRefGoogle Scholar
  20. Soltis DE, Haufler CH, Darrow DC (1983) Amer Fern J 73:9–27Google Scholar
  21. Stuber CW, Goodman MM, Johnson FM (1977) Biochem Genet 15: 383–394CrossRefPubMedGoogle Scholar
  22. Thengane SR, Joshi MS, Kuspe SS, Mascarenhas (1994) Plant Cell Reports 13:222–226CrossRefGoogle Scholar
  23. Vallejos E (1983) In: Tanksley SD and Orton TJ (eds) Isozymes in plant genetics and breeding. Part A, Elvesier, Amsterdam, pp 469–516Google Scholar
  24. Zhong D, Michaux-Feniere N, Coumans M (1995) Plant Cell; Tissue and Organ Culture 41: 91–97Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Tengku Nurhidayah
    • 1
  • Renate Horn
    • 1
  • Thomas Röcher
    • 1
  • Wolfgang Friedt
    • 1
  1. 1.Institut für Pflanzenbau and Pflanzenzüchtung IJustus-Liebig-UniversitätGiessenGermany

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