Plant Cell Reports

, Volume 16, Issue 3–4, pp 153–157 | Cite as

Factors influencing secondary somatic embryogenesis inMalus x domestica Borkh. (cv ‘Gloster 69’)

  • G. Daigny
  • H. Paul
  • R. S. Sangwan
  • B. S. Sangwan-Norreel
Article

Abstract

A procedure for regeneration of apple plants through secondary somatic embryogenesis (SSE) was developed in apple ‘Gloster 69’. Primary somatic embryos were produced from cotyledon-derived cultures of immature zygotic embryos. These somatic embryos were multiplied by secondary somatic embryogenesis (SSE) on media with different Plant Growth Regulator (PGR) combinations. The highest SSE rate (55.5%) was obtained with a combination of NAA (5.3 μM), BAP (0.9 μM) and KIN (0.9 μM) or with TDZ alone (10 μM). In addition, effects of explant source, somatic embryo size, type and concentrations of carbohydrates and gelling agents on SSE were investigated. The optimum SSE (>73%) was obtained by the culture of large size somatic embryos or cotyledon-like structures on medium containing a combination of NAA/BAP/KIN or TDZ (10 μM) alone, maltose (175 mM) and Phytagel (2.8 g/1).

Keywords

Carbohydrate Plant Growth Somatic Embryo Growth Regulator Somatic Embryogenesis 

Abbreviations

NAA

(α-naphthaleneacetic acid

BAP

6-benzylaminopurine

KIN

kinetin

IBA

indolebutyric acid

2,4-D

2,4-dichlorophenoxyacetic acid

TDZ

thidiazuron

PSE

primary somatic embryogenesis

SSE

secondary somatic embryogenesis

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • G. Daigny
    • 1
  • H. Paul
    • 1
  • R. S. Sangwan
    • 1
  • B. S. Sangwan-Norreel
    • 1
  1. 1.Faculté des Sciences, Laboratoire Androgenèse et BiotechnologieUniversité de Picardie-Jules VerneAmiens CedexFrance

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