Plant Cell Reports

, Volume 16, Issue 3–4, pp 137–141 | Cite as

Primary callus as source of totipotent barley (Hordeum vulgare L.) protoplasts

  • Annette Stöldt
  • Xiao-Hui Wang
  • Horst Lörz


Primary callus of barley (Hordeum vulgare L.) derived from scutella (cv. ‘Dissa’) and anthers (cv. ‘Igri’) was used for protoplast isolation and plant regeneration. The protoplasts were embedded in agarose and cultured with nurse cells. The plating efficiency varied from 0.1% to 0.7%. Shoots regenerated from the developing callus. Plantlets were transferred to soil and cultivated in the greenhouse three to five months after protoplast isolation. All plants were normal in morphology, and most of them flowered and set seeds.

Key words

Protoplasts Hordeum vulgare L. nurse culture plant regeneration primary callus 



2,4-dichlorophenoxyacetic acid




Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Biswas GCG, Burkhardt PK, Wünn J, Klöti A, Potrykus I (1994) Plant Cell Rep 13:528–532Google Scholar
  2. Funatsuki H, Lörz H, Lazzeri PA (1992) Plant Sci 85:179–187CrossRefGoogle Scholar
  3. Golds TJ, Babczinky J, Mordhorst AP Koop H-U (1994) Plant Cell Rep 13:188–192CrossRefGoogle Scholar
  4. Jähne A, Lazzeri PA, Jäger-Gussen M, Lörz H (1991a) Theor Appl Genet 82:74–80CrossRefGoogle Scholar
  5. Jähne A, Lazzeri PA, Lörz H (1991b) Plant Cell Rep 10:1–6CrossRefGoogle Scholar
  6. Jain RK, Khera GS, Lee SH, Blackhall NW, Marchant R, Davey MR, Power JB, Cocking EC, Gosal SS (1995) Plant Cell Rep 14:515–519CrossRefGoogle Scholar
  7. Karp A, Wu QS, Steele SH, Jones MGK (1987) Theor Appl Genet 74, 140–146CrossRefGoogle Scholar
  8. Kihara M, Funatsuki H (1995) Plant Sci 106: 115–120CrossRefGoogle Scholar
  9. Kihara M, Funatsuki H (1994) Plant Cell Rep 13: 551–555Google Scholar
  10. Krautwig B, Lörz H (1995) Plant Sci 1ll: 1–10CrossRefGoogle Scholar
  11. Lazzeri PA, Brettschneider R, Lührs, Lörz H (1991) Theor Appl Genet 81: 437–444CrossRefGoogle Scholar
  12. Lörz H, Göbel E, Brown P (1983) Plant Breeding 100:1–25Google Scholar
  13. Lührs R., Lörz H (1988) Planta 175:71–81CrossRefGoogle Scholar
  14. Mordhorst A.P., Lörz H. (1993) J Plant Physiol 142:485–492Google Scholar
  15. Salmenkallio-Martilla M, Kauppinen V (1995) Plant Cell Rep 14:253–256Google Scholar
  16. Shillito RD, Paszkowski J, Potrykus I (1983) Plant Cell Rep 2:244CrossRefGoogle Scholar
  17. Thompson JA, Abdullah R, Cocking EC (1986) Plant Sci 47: 123–133CrossRefGoogle Scholar
  18. Vasil IK (1992) Physiol Plant 85:279–283CrossRefGoogle Scholar
  19. Wang X-H, Lazzeri PA, Lörz H (1992) Theor Appl Genet 85:181–185Google Scholar
  20. Wu C, Zapata FJ, (1992) Plant Sci 86:83–87.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Annette Stöldt
    • 1
  • Xiao-Hui Wang
    • 1
  • Horst Lörz
    • 1
  1. 1.Zentrum für Angewandte Molekularbiologie der Pflanzen (AMP 11), Institut für Allgemeine BotanikUniversität HamburgHamburgGermany

Personalised recommendations