Plant Cell Reports

, Volume 16, Issue 3–4, pp 137–141 | Cite as

Primary callus as source of totipotent barley (Hordeum vulgare L.) protoplasts

  • Annette Stöldt
  • Xiao-Hui Wang
  • Horst Lörz
Article

Summary

Primary callus of barley (Hordeum vulgare L.) derived from scutella (cv. ‘Dissa’) and anthers (cv. ‘Igri’) was used for protoplast isolation and plant regeneration. The protoplasts were embedded in agarose and cultured with nurse cells. The plating efficiency varied from 0.1% to 0.7%. Shoots regenerated from the developing callus. Plantlets were transferred to soil and cultivated in the greenhouse three to five months after protoplast isolation. All plants were normal in morphology, and most of them flowered and set seeds.

Key words

Protoplasts Hordeum vulgare L. nurse culture plant regeneration primary callus 

Abbreviations

2,4-D

2,4-dichlorophenoxyacetic acid

BAP

6-benylaminopurine

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Annette Stöldt
    • 1
  • Xiao-Hui Wang
    • 1
  • Horst Lörz
    • 1
  1. 1.Zentrum für Angewandte Molekularbiologie der Pflanzen (AMP 11), Institut für Allgemeine BotanikUniversität HamburgHamburgGermany

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