Summary
Uptake and respiration of radioactive glucose, mannitol and arabitol were studied during basidiospore germination of the woodrotting mushroomSchizophyllum commune. Glucose uptake was rapid and immediate, depressed at 4° C and unaffected by the protein synthesis antagonist cycloheximide. In contrast, uptake of either mannitol or arabitol exhibited a lag phase while the induction of this process was sensitive to cycloheximide. Prior incubation of basidiospores in unlabelled mannitol induced uptake processes for either labelled mannitol or arabitol. Respiratory rates for either arabitol or mannitol increased markedly upon germination in glucose-asparagine minimal culture medium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aitken, W. B., Niederpruem, D. J.: Ultrastructural changes and biochemical events in basidiospore germination ofSchizophyllum commune. J. Bact.104, 981–988 (1970).
Blumenthal, H. J.: Glycolysis. The fungiI, 229–268 (1965).
—: Glucose catabolism in fungi. Wallerstein Communications1, 171–191 (1968).
Braun, M. L., Niederpruem, D. J.: Erythritol metabolism in wild-type and mutant strains ofSchizophyllum commune. J. Bact.100, 625–634 (1969).
Budd, K., Sussman, A. S., Eilers, F. I.: Glucose-C14 metabolism of dormant and activated ascospores ofNeurospora. J. Bact.91, 551–561 (1966).
Clark, D. S., Wallace, R. H.: Carbohydrate metabolism ofPullularia pullulans. Canad. J. Microbiol.4, 43–54 (1958).
Cochrane, V. W., Berry, S. J., Simon, F. G., Cochrane, J. C., Collins, C. B., Levy, J. A., Holmes, P. K.: Spore germination and carbon metabolism inFusarium solani. III. Carbohydrate respiration in relation to germination. Plant Physiol.38, 533–541 (1963).
Gottlieb, D., Tripathi, R. K.: The physiology of swelling phase of spore germination inPenicillium atrovenetum. Mycologia (N.Y.)60, 571–590 (1968).
Katz, J., Wood, H. G.: The use of glucose-C14 for the evaluation of the pathways of glucose metabolism. J. biol. Chem.235, 2165–2177 (1960).
Maxwell, W. A., Spoerl, E.: Mannitol uptake bySaccharomyces cerevisiae. J. Bact.105, 753–758 (1971).
McKinsey, R. D.: Glucose dissimilation inUstilago maydis. Amer. J. Bot.46, 566–571 (1959).
Mitchell, D., Shaw, M.: Metabolism of glucose-14C, pyruvate-14C, and mannitol-14C byMelampsora lini. II. Conversion to soluble products. Canad. J. Bot.46, 453–460 (1968).
Newburgh, R. W., Cheldelin, V. H.: Oxidation of labelled substrates by dwarf buntTilletia contraversa. Canad. J. Microbiol.5, 415–417 (1959).
Niederpruem, D. J.: Respiration of basidiospores ofSchizophyllum commune. J. Bact.88, 210–215 (1964).
—, Dennen, D. W.: Kinetics, nutrition and inhibitor properties of basidiospore germination inSchizophyllum commune. Arch. Mikrobiol.54, 91–105 (1966).
—, Hafiz, A., Henry, L.: Polyol metabolism in the BasidiomyceteSchizophyllum commune. J. Bact.89, 954–959 (1965).
Niederpruem, D. J., Hunt, S.: Polyols inSchizophyllum commune. Amer. J. Bot.54, 241–245 (1967).
Scheld, H. W., Perry, J. J.: Basidiospore germination in the wood destroying fungusLenzites saepiaria. J. gen. Microbiol.60, 9–21 (1970).
Sussman, A. S., Halvorson, H. O.: Spores. Their dormancy and germination. New York: Harper and Row 1966.
Turel, F. L. M., Ledingham, G. A.: Utilization of labelled substrates by the mycelium and uredospores of flax rust. Canad. J. Microbiol.5, 537–545 (1959).
Williams, P. G., Shaw, M.: Metabolism of glucose-14C, pyruvate-14C and mannitol-14C byMelampsora lini. I. Uptake. Canad. J. Bot.46, 435–440 (1968).
Author information
Authors and Affiliations
Additional information
This research was supported by Public Health Service Grant AI-04603-09 to Donald J. Niederpruem.
Rights and permissions
About this article
Cite this article
Aitken, W.B., Niederpruem, D.J. Isotopic studies of carbohydrate metabolism during basidiospore germination inSchizophyllum commune . Archiv. Mikrobiol. 82, 173–183 (1972). https://doi.org/10.1007/BF01890408
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01890408