Abstract
The ribosomal protein HS23 from the 30S subunit of the extreme halophilicHaloarcula marismortui, belonging to the group of archaea, was isolated either by RP-HLPLC or two-dimensional polyacrylamide gel electrophoresis. The complete amino acid sequence was determined by automated N-terminal microsequencing. The protein consists of 123 residues with a corresponding molecular mass of 12,552 Da as determined by electrospray mass spectroscopy; the pI is 11.04. Homology studies reveal similarities to the eukaryotic ribosomal protein S8 fromHomo sapiens, Rattus norvegicus, Leishmania major, andSaccharomyces cerevisiae.
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Abbreviations
- H. marismortui :
-
Haloarcula marismortui
- PVDF:
-
polyvinylidene difluoride
- PTH:
-
phenylthiohydantoin
- RP-HPLC:
-
reversed-phase high-performance liquid chromatography
- TFA:
-
trifluoro acetic acid
- TP30:
-
total protein mixture from the 30S ribosomal subunit ofH. marismortui
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Engemann, S., Herfurth, E., Briesemeister, U. et al. Amino acid sequence of the ribosomal protein HS23 from the halophilicHaloarcula marismortui and homology studies to other ribosomal proteins. J Protein Chem 14, 189–195 (1995). https://doi.org/10.1007/BF01886759
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DOI: https://doi.org/10.1007/BF01886759