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A simple method for ultrastructural enzyme cytochemistry on small volumes and numbers of isolated cells

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Summary

A simple technique is described for ultrastructural enzyme cytochemistry using small volumes and numbers of single cells. Small aliquots (1–5 μl) of single cells suspended in a glutaraldehyde fixative are incubated on poly-L-lysine-coated glass coverslips. Cells in contact with the coated coverslip become immobilized to that support. All changes of solutions are accomplished without repeated centrifugation.

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Authors and Affiliations

  1. Department of Cell Biology, Neurobiology and Anatomy, The Ohio State University, 43210, Columbus, OH, USA

    J. M. Robinson

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  1. J. M. Robinson
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Robinson, J.M. A simple method for ultrastructural enzyme cytochemistry on small volumes and numbers of isolated cells. Histochem J 23, 10–12 (1991). https://doi.org/10.1007/BF01886502

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  • DOI: https://doi.org/10.1007/BF01886502

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