Summary
After acute carbon monoxide poisoning (inhalation, dogs; perfusion, rats; postmortem, humans) endogenous nickel was cytochemically detected in heart muscle by dimethylglioxime reaction. Dimethylglioxime cytochemical reaction, in each case at the COHb level, was always positive in experiments and in postmortem samples. A nickel ion accumulation in the heart muscle above 30 rel.% of COHb was suggested. A possible role of nickel ion in the pathomechanism of the acute carbon monoxide poisoning was supposed. This dimethylglioxime cytochemical technique is applicable in forensic medical practice, primarily because it is not disturbed by autolysis.
Zusammenfassung
Bei unterschiedlicher Einwirkung von Kohlenmonoxid (Inhalation, Perfusion, post mortem) wurde endogenes Nickel im Herzmuskel histochemisch durch die Dimethyl-Glyoxim-Reaktion nachgewiesen. Die histochemische Reaktion ist für den morphologischen Nachweis ab 30% COHb geeignet. Die Methode ist für die Routinearbeit zu empfehlen, zumal die Reaktion nicht durch Autolyse gestört wird.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Balogh I, Sótonyi P, Somogyi E (1975) Effect of experimental carbon monoxide poisoning on the heart muscle. Acta Morphol Acad Sci Hung 23:165–172
Balogh I (1976) Electron-microscopic investigation of myocardial lesions induced by coal-gas poisoning. Morphol Igazsagugyi Orv Sz 16:85–92
Balogh I, Rubányi G, Somogyi E, Kovách AGB, Sótonyi P, Héjj G (1979) Effect of nickel ions on the perfused rat heart ultrastructure. Morphol Igazsagugyi Orv Sz 19:104–110
Balogh I, Rubányi G (1980) Electron-microscopic localization of nickel in the myocardium, coronary and uterine smooth muscle. In: Spurenelemente, Ni-As. Jena, pp 129–135
Balogh I, Rubányi G, Kovách AGB, Sótonyi P, Somogyi E (1981) Electron-cytochemical detection of nickel ions under pathological conditions in the rat heart. Acta Morphol Acad Sci Hung 29:87–90
Balogh I, Rubányi G, Schulze W, Kovách AGB, Sótonyi P, Somogyi E (1981) Electron-cytochemical studies on changes of the adenylcyclase activity following experimental hypoxia and ischemia in perfused rat hearts. Exp Pathol 20:121–127
Balogh I, Somogyi E, Rubányi G (1982) Nickel cytochemistry: applicability of a new cytochemical technique. Proc XIIth Congr Int Acad Forens Soc Med, Vienna, pp 459–464
Balogh I, Rubányi G, Sótonyi P, Kovách A, Somogyi E (1981) Possibilities for electron-cytochemical detection of nickel ions under certain pathological conditions. In: Elektron-enmikroskopie. Leipzig, pp 160–161
Kovách AGB, Rubányi G, Balogh I (1979) Effect of nickel ions on contractility, metabolism, coronary resistance and ultrastructure of isolated rat heart. Bol Soc Port Cardiol [Suppl 2] 17:269–285
Rubányi G, Balogh I, Somogyi E, Kovách AGB, Sótonyi P (1980) Effect of nickel ions on ultrastructure of isolated perfused rat heart. J Mol Cell Cardiol 12:609–618
Rubányi G (1980) Effect of calcium and nickel ions on oxidative and glycolytic metabolism in the perfused rat heart. Adv Physiol Sci 8:139–147
Somogyi E, Balogh I, Rubányi G, Sótonyi P, Szegedy L (1981) New findings concerning the pathogenesis of acute carbon monoxide (CO) poisoning. Am J Forensic Med Pathol 2:31–39
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Balogh, I., Somogyi, E., Sótonyi, P. et al. Electron-cytochemical detection of endogenous nickel in the myocardium in acute carbon monoxide poisoning. Z Rechtsmed 90, 7–14 (1983). https://doi.org/10.1007/BF01886061
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01886061