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The Histochemical Journal

, Volume 22, Issue 2, pp 95–101 | Cite as

Carboxylic ester hydrolases in mitochondria from rat skeletal muscle

  • S. Kirkeby
  • D. Moe
  • T. Zelander
Papers
  • 41 Downloads

Summary

A mitochondrial pellet, prepared from rat skeletal muscle, contained a number of carboxylic ester hydrolase isoenzymes. The esterases which split α-naphthyl acetate were organophosphate sensitive, whereas two out of three indoxyl acetate hydrolysing enzymes were resistant to both organophosphate and organomercury. The activity of the indoxyl acetate esterases was enhanced by the non-ionic detergents Tween-40 and Lubrol. After freezing, thawing and high speed centrifugation most of the α-naphthyl acetate splitting enzymes were found in the supernatant, indicating that the enzymes are loosely bound to mitochondrial membranes.

Keywords

Acetate Ester Skeletal Muscle Mitochondrial Membrane Hydrolyse Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall Ltd. 1990

Authors and Affiliations

  • S. Kirkeby
    • 1
  • D. Moe
    • 1
  • T. Zelander
    • 1
  1. 1.Institute of General and Oral AnatomyRoyal Dental CollegeCopenhagenDenmark

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