Summary
A dilution plating method estimatedPenicillium urticae Bainier numbers in soil. This method, which used an agar layering technique and a cyclic incubation of 8 hours at room temperature (about 25°C) and 16 hours at 5°C, permitted the differential growth and sporulation in favor ofP. urticae B. over other common soil fungi.
Procedures of extraction, paper chromatography, infrared analysis, and bioassay assayed for accumulated patulin. A combination of these methods routinely estimatedP. urticae B. numbers in soils and authenticated patulin production by selected isolates.
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References
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Additional information
Contribution from the Northern Plains Branch, Soil and Water Conservation Research Division, Agricultural Research Service, USDA, in cooperation with the Nebraska Agricultural Experiment Station, Lincoln. Published as Paper No. 2275, Journal Series, Nebraska Agricultural Experiment Station.
Soil Scientist, USDA, Grand Junction, Colorado (formerly Chemist, USDA, Lincoln, Nebraska); and Microbiologist, USDA, Lincoln, Nebraska, respectively.
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Norstadt, F.A., McCalla, T.M. Penicillium urticae Bainier enumeration in soils. Plant Soil 30, 129–133 (1969). https://doi.org/10.1007/BF01885272
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DOI: https://doi.org/10.1007/BF01885272