Far-infrared radiation effect on the structure and properties of proteins

  • V. M. Govorun
  • V. E. Tretiakov
  • N. N. Tulyakov
  • V. B. Fleurov
  • A. I. Demin
  • A. Yu. Volkov
  • V. A. Batanov
  • A. B. Kapitanov
Article

Abstract

This paper describes the effect of far-infrared radiation on the activity and secondary structure of albumin, alcohol dehydrogenase, peroxidase and trypsin. For the first time the data obtained allowed a conclusion that enzymatic activity changes under the influence of radiation having the wavelength of 90 μm. No disruptures of polypeptide chains or oligomerization of proteins were obtained. It has been shown that albumin secondary structure calculated from circular dichroism spectra changes lineary according to dose of laser emission.

Keywords

Radiation Alcohol Enzymatic Activity Albumin Oligomerization 

Abbreviations

FIR

far-infarred radiation

CD

circular dichroism

ADG

alcohol dehydrohenase

PXD

peroxidase

ANS

1-anilinenaphalene-8-sulfonic acid

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Blackman, C.F., Surles, M.C., and Benane, S.C. (1976) Biol. Effects of Electromagnetic Waves, 1, 406–413.Google Scholar
  2. 2.
    Batanov V.A., Volkov A. Yu., Govorun V.M., Demin A.I., Kapitanov A.B. Timofeev S.V., Fleurov V.B. Preprint IOFAN no. 3, (1989).Google Scholar
  3. 3.
    Zhengyu, Mi (1989) Infrared Phys., 29, 631–636.Google Scholar
  4. 4.
    Batanov V.A., Fleurov V.B., Khlebnikov O.M., Kuzmin K.Yu., Lesnov I.A., Timofeev S.V. Volkov A.Yu. Int. J. of IR & MM Waves, v. 11, p. 435–442 (1989).Google Scholar
  5. 5.
    Vladimirov, Yu.A., and Dobretsov, G.E. Fluorescense probes in investigation of biological membranes (1980) Nauka, Moscow.Google Scholar
  6. 6.
    Robertson, D.E., and Rottenberg, H. (1983) J. Biol. Chem., 258, 11039–11044.Google Scholar
  7. 7.
    Lowry, O.H., Rosebrough, N.I., Farr, A.L., and Randall, R.I. (1951) J. Biol. Chem., 193, 265–275.PubMedGoogle Scholar
  8. 8.
    van Iersel, I., Izn, I.F., and Duine, I.A. (1985) Anal. Biochem., 151, 196–204.Google Scholar
  9. 9.
    Bolotina, I.A., Chehov, V.O., Lugauskas, V.Yu., and Ptisyn, O.B. (1981) Molek. Biol., 15, 167–175.Google Scholar
  10. 10.
    Vladimirov, Y.A., Gorbatenkova, E.A., Paramonov, N.V., and Azizova, O.A. (1988) Free Radical Biology & Medicine, 5, 281–286.Google Scholar

Copyright information

© Plenum Publishing Corporation 1991

Authors and Affiliations

  • V. M. Govorun
    • 1
  • V. E. Tretiakov
    • 1
  • N. N. Tulyakov
    • 1
  • V. B. Fleurov
    • 2
  • A. I. Demin
    • 2
  • A. Yu. Volkov
    • 2
  • V. A. Batanov
    • 2
  • A. B. Kapitanov
    • 1
  1. 1.Institute of Physical-Chemical MedicineMoscowUSSR
  2. 2.Institute of Physics and Technology of the USSR Academy of SciencesMoscowUSSR

Personalised recommendations