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Untersuchung des Histamingehalts im Gehirn der Ratte nach experimentellen Verbrennungen

Examination of the histamine content of the brain in experimental burns injuries in rats

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Summary

Third-degree burns on 20% of body surface were induced on the back skin of rats. After 1, 5, 10, 30 min; 2, 6, 12, 24 hrs and 2, 3, 5, 7 days the histamine content of the brain was determined by a spectro-fluorometric method.

In the brain of control rats 0.44 μg/g free histamine and 0.78 μg/g total histamine were found. The free histamine level in brains ofburned rats showed in the 1st min a rise of 40.9%x, in the 2nd hr 386.4%, on the 24th hr 179.5%, on the 2nd day 295.4%, on the 5th day 502.3%.

In the 1 week interval the average level of thefree-histamine in brains of burned rats was 1.10 + 0.68 μg/g, that of total histamine 2.31 + 1.65 μg/g. In the brain of postmortal burned rats the histamine showed no change as compared with control rats.

Zusammenfassung

An Ratten wurden auf etwa 20% der Körperfläche ausmachenden Arealen der Rückenhaut Verbrennungen III. Grades hervorgerufen und 1, 5, 10 und 30 min. 2, 6, 12 und 24 Std sowie 2, 3, 5 und 7 Tage darauf der Histamingehalt im Gehirn bestimmt.

In der Gehirnsubstanz der Kontrolltiere konnten 0,44 μg/g freies und 0,78 μg/g Gesamthistamin nachgewiesen werden, während in jener derverbrannten Ratten der Gehalt an freiem Histamin in der 1. min um 40,9%, in der 2. Std um 386,4%, in der 24. Std um 179,5%, am 2. Tage um 295,4% und am 5. Tage um 502,3% erhöht war. Der Gesamthistaminwert zeigt einen analogen Anstieg zum freien Histamin und erreicht sein Maximum am 5. Tage mit 629,5%.

Innerhalb des untersuchten einwöchigen Intervalls betrug der in der Hirnsubstanz der verbrennungsverletzten Ratten ermittelte Gehalt anfreiem Histamin im Mittel 1,19±0,69 μg/g und die Gesamthistaminmenge im Mittel 2,31±1,65 μg/g.

In der Gehirnsubstanz der postmortal lädierten Ratten blieb der Histamingehalt im Verhältnis zu dem der Kontrolltiere unverändert.

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Authors and Affiliations

  1. Institut für gerichtliche Medizin der Universität Kossuth Lajos sugárút 40, H-Szeged, Ungarn

    I. Gyula Fazekas, Ferenc Kósa, Elisabet Virágos-Kis & András Basch

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  1. I. Gyula Fazekas
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  2. Ferenc Kósa
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  3. Elisabet Virágos-Kis
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  4. András Basch
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Gyula Fazekas, I., Kósa, F., Virágos-Kis, E. et al. Untersuchung des Histamingehalts im Gehirn der Ratte nach experimentellen Verbrennungen. Z Rechtsmed 73, 149–157 (1973). https://doi.org/10.1007/BF01882339

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